Detailed information of TA system TA075027

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 302383..302979
• Replicon: chromosome
• Accession: NZ_CP020528
• Organism: Enterobacter cloacae strain 174

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: AXJ76_RS01480
• Protein ID: WP_045350767.1
• Coordinates: 302677..302979 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: AXJ76_RS01475
• Protein ID: WP_032610152.1
• Coordinates: 302383..302670 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXJ76_RS01470	300755..302386	+	1632	WP_003860350.1	Na/Pi cotransporter family protein	-
AXJ76_RS01475	302383..302670	-	288	WP_032610152.1	putative addiction module antidote protein	Antitoxin
AXJ76_RS01480	302677..302979	-	303	WP_045350767.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AXJ76_RS01485	303177..304049	+	873	WP_045346556.1	23S rRNA pseudouridine(2604) synthase RluF	-
AXJ76_RS01490	304050..304322	-	273	WP_003860346.1	DUF3811 domain-containing protein	-
AXJ76_RS01495	304373..305317	-	945	WP_045350764.1	ketopantoate/pantoate/pantothenate transporter PanS	-
AXJ76_RS01500	305411..306760	-	1350	WP_045337031.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11428.16 Da
• Isoelectric Point: 9.2022

>T75027 WP_045350767.1 NZ_CP020528:c302979-302677 [Enterobacter cloacae]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRVYFKDQGNCIIVLLCGGDKS
SQARDILMAEMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T75027 NZ_CP020528:c302979-302677 [Enterobacter cloacae]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAGAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10072.60 Da
• Isoelectric Point: 5.7807

>AT75027 WP_032610152.1 NZ_CP020528:c302670-302383 [Enterobacter cloacae]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISLQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPSGD

Nucleotide

• Length: 288 bp

>AT75027 NZ_CP020528:c302670-302383 [Enterobacter cloacae]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCTGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA