Detailed information of TA system TA075025

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4760734..4761260
• Replicon: chromosome
• Accession: NZ_CP020525
• Organism: Enterobacter cloacae strain 109

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: AO413_RS23645
• Protein ID: WP_000323025.1
• Coordinates: 4760973..4761260 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: AO413_RS23640
• Protein ID: WP_000534858.1
• Coordinates: 4760734..4760973 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AO413_RS23620	4757062..4758300	+	1239	WP_003030308.1	IS110 family transposase	-
AO413_RS23625	4758776..4759348	+	573	WP_001515348.1	cytochrome b/b6 domain-containing protein	-
AO413_RS23630	4759548..4760471	+	924	WP_000167917.1	cation diffusion facilitator family transporter	-
AO413_RS23640	4760734..4760973	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
AO413_RS23645	4760973..4761260	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
AO413_RS23650	4761331..4761489	+	159	WP_064758473.1	type I toxin-antitoxin system Hok family toxin	-
AO413_RS23660	4761723..4761929	-	207	WP_044255297.1	hypothetical protein	-
AO413_RS23665	4761969..4762319	-	351	WP_032610485.1	hypothetical protein	-
AO413_RS23670	4762343..4762912	-	570	WP_032610483.1	hypothetical protein	-
AO413_RS23675	4762957..4763289	-	333	WP_032610481.1	DUF5417 domain-containing protein	-
AO413_RS23680	4763414..4763662	-	249	WP_050596976.1	hypothetical protein	-
AO413_RS23685	4763722..4764276	-	555	WP_015063016.1	hypothetical protein	-
AO413_RS23690	4764462..4764743	-	282	WP_032610524.1	hypothetical protein	-
AO413_RS23695	4764861..4765277	-	417	WP_032610480.1	hypothetical protein	-
AO413_RS23700	4765277..4765531	-	255	WP_032610479.1	hypothetical protein	-
AO413_RS23705	4765542..4765925	-	384	WP_050596975.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4728027..4805181	77154

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T75025 WP_000323025.1 NZ_CP020525:4760973-4761260 [Enterobacter cloacae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T75025 NZ_CP020525:4760973-4761260 [Enterobacter cloacae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT75025 WP_000534858.1 NZ_CP020525:4760734-4760973 [Enterobacter cloacae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT75025 NZ_CP020525:4760734-4760973 [Enterobacter cloacae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8