Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 4643351..4644974 Replicon chromosome
Accession NZ_CP020525
Organism Enterobacter cloacae strain 109

Toxin (Protein)


Gene name hipA Uniprot ID A0A7D6U035
Locus tag AO413_RS22955 Protein ID WP_020833804.1
Coordinates 4643649..4644974 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A7W3GWM9
Locus tag AO413_RS22950 Protein ID WP_058656924.1
Coordinates 4643351..4643644 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AO413_RS26575 4638517..4638696 + 180 WP_071685142.1 hypothetical protein -
AO413_RS22930 4638862..4639518 + 657 WP_059509512.1 trypsin-like serine protease -
AO413_RS22935 4639721..4640218 + 498 WP_000340139.1 membrane protein -
AO413_RS22940 4640276..4641516 - 1241 Protein_4370 IS3 family transposase -
AO413_RS22945 4641598..4642950 + 1353 WP_058656920.1 hypothetical protein -
AO413_RS22950 4643351..4643644 + 294 WP_058656924.1 helix-turn-helix transcriptional regulator Antitoxin
AO413_RS22955 4643649..4644974 + 1326 WP_020833804.1 type II toxin-antitoxin system HipA family toxin Toxin
AO413_RS22960 4645035..4645241 + 207 WP_000134171.1 hypothetical protein -
AO413_RS22965 4645411..4645905 + 495 WP_071685143.1 hypothetical protein -
AO413_RS22970 4645905..4646222 + 318 WP_058656922.1 hypothetical protein -
AO413_RS22975 4646219..4646896 + 678 WP_148116556.1 IS21-like element helper ATPase IstB -
AO413_RS22980 4647095..4648099 - 1005 WP_000427614.1 IS110-like element IS5075 family transposase -
AO413_RS22985 4648178..4648846 - 669 WP_058678057.1 glutathione S-transferase N-terminal domain-containing protein -
AO413_RS22990 4648979..4649518 - 540 WP_004099036.1 peptide-methionine (S)-S-oxide reductase MsrA -
AO413_RS22995 4649520..4649963 - 444 WP_004099035.1 peptide-methionine (R)-S-oxide reductase MsrB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4461429..4707913 246484
- flank IS/Tn - - 4640276..4641163 887


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50307.23 Da        Isoelectric Point: 6.0252

>T75023 WP_020833804.1 NZ_CP020525:4643649-4644974 [Enterobacter cloacae]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLDQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T75023 NZ_CP020525:4643649-4644974 [Enterobacter cloacae]
ATGAGCCGGAAACAGCAACGTCTGGTAATTTGGATGAACGGCATCAAAGTCGGGTACTGGGAAAAAAGCAAAGGGGTAGA
CAGCTTAGAATACCTTCCTGAATGGGTTGCTGACGAACAGGGAAGGCCGCTGTCATTATCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTCCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTGCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACTGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGAGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGACCAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAGACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCGTTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAGGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAATTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCAAACTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCTTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11024.41 Da        Isoelectric Point: 9.9492

>AT75023 WP_058656924.1 NZ_CP020525:4643351-4643644 [Enterobacter cloacae]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSVLGVKISFSSATTSSERK
QTEEIYKFNSPARQEDW

Download         Length: 294 bp

>AT75023 NZ_CP020525:4643351-4643644 [Enterobacter cloacae]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGTTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGTGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCTGTCCTGGGTGTAAAAATCAGCTTCTCCTCGGCAACCACTTCTTCAGAGAGGAAG
CAGACGGAAGAAATATATAAATTTAATTCACCTGCACGACAGGAGGATTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6U035

Antitoxin

Source ID Structure
AlphaFold DB A0A7W3GWM9

References