Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3529151..3529890 Replicon chromosome
Accession NZ_CP020525
Organism Enterobacter cloacae strain 109

Toxin (Protein)


Gene name yeeV Uniprot ID A0A431SEP0
Locus tag AO413_RS17295 Protein ID WP_022649088.1
Coordinates 3529510..3529890 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A431SER1
Locus tag AO413_RS17290 Protein ID WP_022649087.1
Coordinates 3529151..3529477 (+) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AO413_RS17275 3525045..3527705 - 2661 WP_022649084.1 hypothetical protein -
AO413_RS17280 3528239..3528658 + 420 Protein_3298 antirestriction protein -
AO413_RS17285 3528673..3529140 + 468 WP_022649086.1 DNA repair protein RadC -
AO413_RS17290 3529151..3529477 + 327 WP_022649087.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
AO413_RS17295 3529510..3529890 + 381 WP_022649088.1 TA system toxin CbtA family protein Toxin
AO413_RS17300 3529887..3530114 + 228 WP_022649089.1 hypothetical protein -
AO413_RS17305 3530506..3530799 + 294 WP_022649090.1 hypothetical protein -
AO413_RS17310 3531143..3532459 - 1317 WP_022649091.1 amidase -
AO413_RS17315 3532514..3533809 - 1296 WP_022649092.1 histidinol dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3506042..3530799 24757


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-110)

Antitoxin

Antitoxin CbeA

(5-103)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13850.66 Da        Isoelectric Point: 5.8788

>T75020 WP_022649088.1 NZ_CP020525:3529510-3529890 [Enterobacter cloacae]
MQTLPLPEPRAAEPCPSPVQVWQLLLTHLLDKHYGLTLNDTPFGDDGVIQAHINAGISLCDAVNFIVERYELLRTDHSSS
SLTEHSSLIGAIDILRAHRATGLIVGSGYSTITRITTGQYREDKTQ

Download         Length: 381 bp

>T75020 NZ_CP020525:3529510-3529890 [Enterobacter cloacae]
ATGCAAACTTTACCGTTACCCGAACCTCGGGCGGCTGAGCCATGTCCGTCGCCCGTGCAGGTCTGGCAACTCCTGCTCAC
GCATCTGCTGGATAAGCATTATGGTCTCACACTCAATGACACGCCGTTTGGCGACGACGGCGTAATTCAGGCGCACATCA
ACGCCGGTATCTCTCTGTGCGACGCCGTCAACTTCATTGTGGAAAGGTATGAACTGTTGCGCACTGACCACAGCAGCTCC
AGCCTCACCGAGCACTCTTCACTGATTGGTGCCATCGACATCCTGCGTGCCCACCGTGCAACCGGCCTGATAGTGGGCAG
CGGCTACAGCACCATCACCCGCATCACAACGGGCCAGTATCGGGAGGACAAAACACAGTGA

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 11923.47 Da        Isoelectric Point: 5.8762

>AT75020 WP_022649087.1 NZ_CP020525:3529151-3529477 [Enterobacter cloacae]
MHSEQWGLTCGKTPCFSAKLVQEGNRLHFLADRAEFSGDLSDDEALRLDQAFPVILKQLEQMLSAGELNPRHQHRVTLHL
NGLTCEADTLGSHGYLYIAIYPSAVATG

Download         Length: 327 bp

>AT75020 NZ_CP020525:3529151-3529477 [Enterobacter cloacae]
ATGCATTCTGAACAATGGGGACTGACGTGCGGTAAAACGCCGTGCTTCAGTGCCAAGCTGGTGCAGGAAGGCAACCGGCT
GCACTTTCTAGCTGACCGAGCAGAGTTTAGTGGCGACTTGAGCGACGATGAGGCACTGCGCCTCGACCAGGCTTTTCCGG
TGATATTGAAGCAGCTGGAACAAATGCTTTCAGCCGGTGAGCTGAATCCCCGCCATCAGCATCGCGTCACTCTGCATCTT
AACGGGCTAACCTGCGAAGCCGACACGCTCGGTTCGCACGGCTACCTTTACATCGCGATTTATCCCTCAGCGGTAGCTAC
CGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A431SEP0

Antitoxin

Source ID Structure
AlphaFold DB A0A431SER1

References