Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2223261..2224000 Replicon chromosome
Accession NZ_CP020525
Organism Enterobacter cloacae strain 109

Toxin (Protein)


Gene name tacT Uniprot ID A0A3S0FZ61
Locus tag AO413_RS10805 Protein ID WP_022648141.1
Coordinates 2223261..2223746 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A837FCR9
Locus tag AO413_RS10810 Protein ID WP_003857131.1
Coordinates 2223734..2224000 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AO413_RS10780 2219417..2220439 + 1023 WP_022648135.1 alpha/beta hydrolase -
AO413_RS10785 2220535..2221299 + 765 WP_022648136.1 SDR family oxidoreductase -
AO413_RS10790 2221948..2222229 + 282 Protein_2052 DUF4225 domain-containing protein -
AO413_RS10795 2222204..2222533 - 330 WP_022648139.1 hypothetical protein -
AO413_RS10800 2222666..2223235 + 570 WP_022648140.1 hypothetical protein -
AO413_RS10805 2223261..2223746 - 486 WP_022648141.1 GNAT family N-acetyltransferase Toxin
AO413_RS10810 2223734..2224000 - 267 WP_003857131.1 DUF1778 domain-containing protein Antitoxin
AO413_RS10815 2224064..2224993 - 930 WP_022648142.1 LysR family transcriptional regulator -
AO413_RS10820 2225123..2226511 + 1389 WP_022648143.1 MFS transporter -
AO413_RS10825 2226490..2227047 - 558 WP_022648144.1 OmpH family outer membrane protein -
AO413_RS10830 2227168..2228304 - 1137 WP_022648145.1 type 1 fimbrial protein -
AO413_RS10835 2228328..2228852 - 525 WP_087878309.1 fimbrial protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(58-151)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17558.24 Da        Isoelectric Point: 9.6275

>T75011 WP_022648141.1 NZ_CP020525:c2223746-2223261 [Enterobacter cloacae]
VGRVTPPEPLSSVHQLAEFISGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSIRGNGLGADLLQDAVLRCYRVAENIGVRAIMVHALTEDAKAFYIHHGFKASQTQERTLFLRLP
Q

Download         Length: 486 bp

>T75011 NZ_CP020525:c2223746-2223261 [Enterobacter cloacae]
GTGGGACGTGTAACCCCCCCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCATCAGTGGAGAGGCCGTGCTTGA
CGAATGGTTAAAACAAAGAGGTCTGAAAAACCAGGCGCTTGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACAA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGGAAGCGACAGGTAATTTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCTGTCGATGTCTCAATACGCGGAAATGGGTTGGGAGCCGATTT
ACTCCAGGATGCGGTTTTACGCTGTTATCGGGTAGCAGAGAATATTGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGACGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10090.50 Da        Isoelectric Point: 4.3837

>AT75011 WP_003857131.1 NZ_CP020525:c2224000-2223734 [Enterobacter cloacae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Download         Length: 267 bp

>AT75011 NZ_CP020525:c2224000-2223734 [Enterobacter cloacae]
ATGAAATCAGATGTTCAACTCAATCTTAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACTGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAAAATGTGATCCTCGATCGGCGTGTATTCAATT
TTAACGATGAACAGTATGCAGAGTTCATTGATATGCTTGATGCCCCGGTCGCCGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S0FZ61

Antitoxin

Source ID Structure
AlphaFold DB A0A837FCR9

References