Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1080278..1080899 Replicon chromosome
Accession NZ_CP020525
Organism Enterobacter cloacae strain 109

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag AO413_RS05240 Protein ID WP_015571250.1
Coordinates 1080278..1080496 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag AO413_RS05245 Protein ID WP_006809850.1
Coordinates 1080525..1080899 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AO413_RS05210 1076290..1076550 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
AO413_RS05215 1076553..1076693 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
AO413_RS05220 1076690..1077400 - 711 WP_022647267.1 GNAT family N-acetyltransferase -
AO413_RS05225 1077503..1078963 + 1461 WP_022647268.1 PLP-dependent aminotransferase family protein -
AO413_RS05230 1078935..1079402 - 468 WP_022647269.1 hypothetical protein -
AO413_RS05235 1079519..1080070 - 552 WP_022647270.1 maltose O-acetyltransferase -
AO413_RS05240 1080278..1080496 - 219 WP_015571250.1 hemolysin expression modulator Hha Toxin
AO413_RS05245 1080525..1080899 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
AO413_RS05250 1081409..1084555 - 3147 WP_022647271.1 multidrug efflux RND transporter permease subunit -
AO413_RS05255 1084578..1085771 - 1194 WP_022647272.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T75010 WP_015571250.1 NZ_CP020525:c1080496-1080278 [Enterobacter cloacae]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T75010 NZ_CP020525:c1080496-1080278 [Enterobacter cloacae]
ATGTCTGATAAACCATTAACTAAAGTCGATTATTTGATGCGCCTGCGACGCTGTCAGTCAATTGACACGCTCGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAAC
TGACCATGAATAAACTCTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT75010 WP_006809850.1 NZ_CP020525:c1080899-1080525 [Enterobacter cloacae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT75010 NZ_CP020525:c1080899-1080525 [Enterobacter cloacae]
TTGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAATGAGCTGATCGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACGAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References