Detailed information of TA system TA075007

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 24555..25080
• Replicon: plasmid unnamed2
• Accession: NZ_CP020523
• Organism: Escherichia coli strain 190

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: J5VBT8
• Locus tag: A9X72_RS26330
• Protein ID: WP_004197633.1
• Coordinates: 24775..25080 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: A9X72_RS26325
• Protein ID: WP_004197642.1
• Coordinates: 24555..24773 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9X72_RS26270	19661..20656	+	996	WP_019725651.1	hypothetical protein	-
A9X72_RS26275	20729..21052	+	324	WP_019725650.1	hypothetical protein	-
A9X72_RS28295	21486..21680	+	195	WP_019725033.1	hypothetical protein	-
A9X72_RS26285	21724..21954	-	231	WP_019725034.1	hypothetical protein	-
A9X72_RS26290	21968..22171	-	204	WP_019725040.1	hemolysin expression modulator Hha	-
A9X72_RS26295	22205..22573	-	369	WP_015065502.1	hypothetical protein	-
A9X72_RS26300	22617..23111	-	495	WP_009310053.1	hypothetical protein	-
A9X72_RS26305	23142..23714	-	573	WP_015065504.1	hypothetical protein	-
A9X72_RS26310	23711..23959	-	249	WP_019725036.1	hypothetical protein	-
A9X72_RS26325	24555..24773	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
A9X72_RS26330	24775..25080	+	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	Toxin
A9X72_RS26335	25249..25650	+	402	WP_072199347.1	hypothetical protein	-
A9X72_RS26340	25677..26000	+	324	WP_004197641.1	hypothetical protein	-
A9X72_RS26345	25997..27013	+	1017	WP_004197639.1	hypothetical protein	-
A9X72_RS26350	27211..28005	+	795	WP_004197635.1	site-specific integrase	-
A9X72_RS26355	28541..28720	-	180	WP_006788217.1	hypothetical protein	-
A9X72_RS26360	28840..29466	-	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qnrB1 / aac(6')-Ib-cr / blaOXA-1 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / dfrA14	-	1..113880	113880

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11585.27 Da
• Isoelectric Point: 6.4661

>T75007 WP_004197633.1 NZ_CP020523:24775-25080 [Escherichia coli]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T75007 NZ_CP020523:24775-25080 [Escherichia coli]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT75007 WP_004197642.1 NZ_CP020523:24555-24773 [Escherichia coli]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT75007 NZ_CP020523:24555-24773 [Escherichia coli]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A2A5MCG5