Detailed information of TA system TA075006

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 69843..70444
• Replicon: plasmid unnamed3
• Accession: NZ_CP020522
• Organism: Escherichia coli strain 190

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: A9X72_RS25990
• Protein ID: WP_001216045.1
• Coordinates: 70064..70444 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: A9X72_RS25985
• Protein ID: WP_001190712.1
• Coordinates: 69843..70064 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9X72_RS25940	64994..65902	+	909	WP_063816972.1	DUF551 domain-containing protein	-
A9X72_RS25945	65985..66218	+	234	WP_000516537.1	hypothetical protein	-
A9X72_RS25950	66397..66690	+	294	WP_050153512.1	hypothetical protein	-
A9X72_RS25955	66697..67071	+	375	WP_000988658.1	hypothetical protein	-
A9X72_RS25960	67053..67985	+	933	WP_000057449.1	hypothetical protein	-
A9X72_RS25965	67982..68344	+	363	WP_001261544.1	hypothetical protein	-
A9X72_RS28485	68419..68556	-	138	WP_000123562.1	hypothetical protein	-
A9X72_RS25975	69006..69257	+	252	WP_001377386.1	DNA polymerase III subunit theta	-
A9X72_RS25980	69381..69770	+	390	WP_000506726.1	DNA repair protein	-
A9X72_RS25985	69843..70064	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
A9X72_RS25990	70064..70444	+	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
A9X72_RS25995	70449..70628	+	180	WP_000113019.1	hypothetical protein	-
A9X72_RS26000	70656..71699	+	1044	WP_086611675.1	DUF968 domain-containing protein	-
A9X72_RS26005	71788..72240	+	453	WP_001326849.1	late promoter-activating protein	-
A9X72_RS26010	72326..73519	+	1194	WP_086611672.1	terminase	-
A9X72_RS26015	73519..75003	+	1485	WP_000124150.1	terminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..95146	95146

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T75006 WP_001216045.1 NZ_CP020522:70064-70444 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T75006 NZ_CP020522:70064-70444 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT75006 WP_001190712.1 NZ_CP020522:69843-70064 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT75006 NZ_CP020522:69843-70064 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6