Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 69843..70444 Replicon plasmid unnamed3
Accession NZ_CP020522
Organism Escherichia coli strain 190

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag A9X72_RS25990 Protein ID WP_001216045.1
Coordinates 70064..70444 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag A9X72_RS25985 Protein ID WP_001190712.1
Coordinates 69843..70064 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9X72_RS25940 64994..65902 + 909 WP_063816972.1 DUF551 domain-containing protein -
A9X72_RS25945 65985..66218 + 234 WP_000516537.1 hypothetical protein -
A9X72_RS25950 66397..66690 + 294 WP_050153512.1 hypothetical protein -
A9X72_RS25955 66697..67071 + 375 WP_000988658.1 hypothetical protein -
A9X72_RS25960 67053..67985 + 933 WP_000057449.1 hypothetical protein -
A9X72_RS25965 67982..68344 + 363 WP_001261544.1 hypothetical protein -
A9X72_RS28485 68419..68556 - 138 WP_000123562.1 hypothetical protein -
A9X72_RS25975 69006..69257 + 252 WP_001377386.1 DNA polymerase III subunit theta -
A9X72_RS25980 69381..69770 + 390 WP_000506726.1 DNA repair protein -
A9X72_RS25985 69843..70064 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
A9X72_RS25990 70064..70444 + 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
A9X72_RS25995 70449..70628 + 180 WP_000113019.1 hypothetical protein -
A9X72_RS26000 70656..71699 + 1044 WP_086611675.1 DUF968 domain-containing protein -
A9X72_RS26005 71788..72240 + 453 WP_001326849.1 late promoter-activating protein -
A9X72_RS26010 72326..73519 + 1194 WP_086611672.1 terminase -
A9X72_RS26015 73519..75003 + 1485 WP_000124150.1 terminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..95146 95146


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T75006 WP_001216045.1 NZ_CP020522:70064-70444 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T75006 NZ_CP020522:70064-70444 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT75006 WP_001190712.1 NZ_CP020522:69843-70064 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT75006 NZ_CP020522:69843-70064 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References