Detailed information of TA system TA075004

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4207280..4208094
• Replicon: chromosome
• Accession: NZ_CP020520
• Organism: Escherichia coli strain 190

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: A9X72_RS21875
• Protein ID: WP_001054376.1
• Coordinates: 4207280..4207537 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: A9X72_RS21880
• Protein ID: WP_001309181.1
• Coordinates: 4207549..4208094 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9X72_RS21845	4202568..4203674	+	1107	WP_023568191.1	N-acetylneuraminate epimerase	-
A9X72_RS21850	4203739..4204719	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
A9X72_RS21855	4205302..4206542	-	1241	Protein_4011	helicase YjhR	-
A9X72_RS21865	4206658..4206903	+	246	Protein_4012	GNAT family N-acetyltransferase	-
A9X72_RS21875	4207280..4207537	+	258	WP_001054376.1	hypothetical protein	Toxin
A9X72_RS21880	4207549..4208094	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
A9X72_RS21885	4208150..4208896	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
A9X72_RS21890	4209065..4209283	+	219	Protein_4016	hypothetical protein	-
A9X72_RS28450	4209321..4209437	+	117	Protein_4017	VOC family protein	-
A9X72_RS21895	4209682..4210803	+	1122	WP_000010829.1	M42 family metallopeptidase	-
A9X72_RS21900	4210800..4211078	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
A9X72_RS21905	4211090..4212403	+	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	4197067..4216318	19251

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T75004 WP_001054376.1 NZ_CP020520:4207280-4207537 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T75004 NZ_CP020520:4207280-4207537 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT75004 WP_001309181.1 NZ_CP020520:4207549-4208094 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT75004 NZ_CP020520:4207549-4208094 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8