Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3596561..3597179 Replicon chromosome
Accession NZ_CP020520
Organism Escherichia coli strain 190

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag A9X72_RS18710 Protein ID WP_001291435.1
Coordinates 3596961..3597179 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag A9X72_RS18705 Protein ID WP_000344800.1
Coordinates 3596561..3596935 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9X72_RS18695 3591650..3592843 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
A9X72_RS18700 3592866..3596015 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
A9X72_RS18705 3596561..3596935 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
A9X72_RS18710 3596961..3597179 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
A9X72_RS18715 3597351..3597902 + 552 WP_000102568.1 maltose O-acetyltransferase -
A9X72_RS18720 3598018..3598488 + 471 WP_000136192.1 YlaC family protein -
A9X72_RS18725 3598652..3600202 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
A9X72_RS18730 3600244..3600597 - 354 WP_000878140.1 DUF1428 domain-containing protein -
A9X72_RS18740 3600976..3601287 + 312 WP_000409911.1 MGMT family protein -
A9X72_RS18745 3601318..3601890 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T75003 WP_001291435.1 NZ_CP020520:3596961-3597179 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T75003 NZ_CP020520:3596961-3597179 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT75003 WP_000344800.1 NZ_CP020520:3596561-3596935 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT75003 NZ_CP020520:3596561-3596935 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References