Detailed information of TA system TA074992

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1357208..1357833
• Replicon: chromosome
• Accession: NZ_CP020520
• Organism: Escherichia coli strain 190

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A9X72_RS07060
• Protein ID: WP_000911330.1
• Coordinates: 1357435..1357833 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: A9X72_RS07055
• Protein ID: WP_000450524.1
• Coordinates: 1357208..1357435 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9X72_RS07030	1353011..1353481	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
A9X72_RS07035	1353481..1354053	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
A9X72_RS07040	1354199..1355077	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
A9X72_RS07045	1355094..1356128	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
A9X72_RS07050	1356341..1357054	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
A9X72_RS07055	1357208..1357435	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
A9X72_RS07060	1357435..1357833	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
A9X72_RS07065	1357980..1358843	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
A9X72_RS07070	1358858..1360873	+	2016	WP_069354268.1	tRNA cytosine(34) acetyltransferase TmcA	-
A9X72_RS07075	1360947..1361645	+	699	WP_000679812.1	esterase	-
A9X72_RS07080	1361755..1361955	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T74992 WP_000911330.1 NZ_CP020520:1357435-1357833 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T74992 NZ_CP020520:1357435-1357833 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT74992 WP_000450524.1 NZ_CP020520:1357208-1357435 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT74992 NZ_CP020520:1357208-1357435 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33