Detailed information of TA system TA074986

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 284294..285094
• Replicon: chromosome
• Accession: NZ_CP020520
• Organism: Escherichia coli strain 190

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: F4NNI0
• Locus tag: A9X72_RS01415
• Protein ID: WP_000342449.1
• Coordinates: 284567..285094 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4NNI1
• Locus tag: A9X72_RS01410
• Protein ID: WP_001277108.1
• Coordinates: 284294..284560 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9X72_RS01385	279951..280619	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
A9X72_RS01390	280612..281670	+	1059	WP_001042003.1	permease-like cell division protein FtsX	-
A9X72_RS01395	281915..282769	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
A9X72_RS01405	283041..284144	+	1104	WP_001350438.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
A9X72_RS01410	284294..284560	+	267	WP_001277108.1	DUF1778 domain-containing protein	Antitoxin
A9X72_RS01415	284567..285094	+	528	WP_000342449.1	GNAT family N-acetyltransferase	Toxin
A9X72_RS01420	285091..285474	-	384	WP_000778781.1	aspartate 1-decarboxylase autocleavage activator PanM	-
A9X72_RS01425	285898..287007	+	1110	WP_000827696.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
A9X72_RS01430	287055..287981	+	927	WP_000003004.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
A9X72_RS01435	287978..289255	+	1278	WP_000803799.1	branched chain amino acid ABC transporter permease LivM	-
A9X72_RS01440	289252..290019	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19691.70 Da
• Isoelectric Point: 7.7457

>T74986 WP_000342449.1 NZ_CP020520:284567-285094 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T74986 NZ_CP020520:284567-285094 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9910.31 Da
• Isoelectric Point: 10.0863

>AT74986 WP_001277108.1 NZ_CP020520:284294-284560 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT74986 NZ_CP020520:284294-284560 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: A0A829CLZ4

Antitoxin

• AlphaFold DB: A0A829CN24