Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 30354..30955 Replicon plasmid unnamed1
Accession NZ_CP020519
Organism Escherichia coli strain 222

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag A9P13_RS28005 Protein ID WP_001216034.1
Coordinates 30575..30955 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag A9P13_RS28000 Protein ID WP_001190712.1
Coordinates 30354..30575 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9P13_RS27990 27347..28618 - 1272 WP_023142242.1 restriction endonuclease subunit S -
A9P13_RS27995 28615..30171 - 1557 WP_001553856.1 type I restriction-modification system subunit M -
A9P13_RS28000 30354..30575 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
A9P13_RS28005 30575..30955 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
A9P13_RS28010 30960..31139 + 180 WP_001513661.1 hypothetical protein -
A9P13_RS28015 31167..31526 + 360 WP_001513660.1 hypothetical protein -
A9P13_RS28020 31450..31863 + 414 Protein_33 DDE-type integrase/transposase/recombinase -
A9P13_RS28030 32659..33636 + 978 WP_000361610.1 RepB family plasmid replication initiator protein -
A9P13_RS28035 33921..34661 - 741 WP_001066942.1 tyrosine-type recombinase/integrase -
A9P13_RS28040 34782..34964 - 183 WP_042065278.1 hypothetical protein -
A9P13_RS28050 35307..35614 - 308 Protein_37 type II toxin-antitoxin system ParD family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / dfrA17 / aadA5 / qacE / sul1 / blaTEM-1B / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / aac(3)-IId - 1..120667 120667
- flank IS/Tn - - 31489..31863 374


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T74977 WP_001216034.1 NZ_CP020519:30575-30955 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T74977 NZ_CP020519:30575-30955 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTAGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT74977 WP_001190712.1 NZ_CP020519:30354-30575 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT74977 NZ_CP020519:30354-30575 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References