Detailed information of TA system TA074964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4385820..4386634
• Replicon: chromosome
• Accession: NZ_CP020516
• Organism: Escherichia coli strain 222

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: A9P13_RS22665
• Protein ID: WP_001054376.1
• Coordinates: 4385820..4386077 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: A9P13_RS22670
• Protein ID: WP_001309181.1
• Coordinates: 4386089..4386634 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9P13_RS22635	4381108..4382214	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
A9P13_RS22640	4382279..4383259	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
A9P13_RS22645	4383842..4385082	-	1241	Protein_4148	helicase YjhR	-
A9P13_RS22655	4385198..4385443	+	246	Protein_4149	GNAT family N-acetyltransferase	-
A9P13_RS22665	4385820..4386077	+	258	WP_001054376.1	hypothetical protein	Toxin
A9P13_RS22670	4386089..4386634	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
A9P13_RS22675	4386690..4387436	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
A9P13_RS22680	4387605..4387823	+	219	Protein_4153	hypothetical protein	-
A9P13_RS28980	4387861..4387977	+	117	Protein_4154	VOC family protein	-
A9P13_RS22685	4388222..4389343	+	1122	WP_000010829.1	M42 family metallopeptidase	-
A9P13_RS22690	4389340..4389618	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
A9P13_RS22695	4389630..4390943	+	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	4375607..4394858	19251

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T74964 WP_001054376.1 NZ_CP020516:4385820-4386077 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T74964 NZ_CP020516:4385820-4386077 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT74964 WP_001309181.1 NZ_CP020516:4386089-4386634 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT74964 NZ_CP020516:4386089-4386634 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8