Detailed information of TA system TA074963

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4222980..4223467
• Replicon: chromosome
• Accession: NZ_CP020516
• Organism: Escherichia coli strain 222

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: A9P13_RS21830
• Protein ID: WP_001520461.1
• Coordinates: 4222980..4223231 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A2T1MJK4
• Locus tag: A9P13_RS21835
• Protein ID: WP_001520458.1
• Coordinates: 4223234..4223467 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9P13_RS21805	4218592..4219878	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
A9P13_RS21810	4219878..4220867	+	990	WP_106504504.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
A9P13_RS21815	4220864..4221685	+	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
A9P13_RS21820	4221688..4222065	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
A9P13_RS21825	4222072..4222920	+	849	WP_000257196.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
A9P13_RS21830	4222980..4223231	-	252	WP_001520461.1	CcdB family protein	Toxin
A9P13_RS21835	4223234..4223467	-	234	WP_001520458.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
A9P13_RS21840	4223553..4224032	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
A9P13_RS21845	4224223..4226085	-	1863	WP_000377113.1	glutathione-regulated potassium-efflux system protein KefC	-
A9P13_RS21850	4226078..4226608	-	531	WP_021522993.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
A9P13_RS21855	4226716..4228047	-	1332	WP_001520455.1	MFS transporter	-
A9P13_RS21860	4228106..4228393	-	288	WP_000203747.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-82)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9482.12 Da
• Isoelectric Point: 10.4134

>T74963 WP_001520461.1 NZ_CP020516:c4223231-4222980 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKF

Nucleotide

• Length: 252 bp

>T74963 NZ_CP020516:c4223231-4222980 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATTCTTTTACTGGTAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8560.61 Da
• Isoelectric Point: 5.8062

>AT74963 WP_001520458.1 NZ_CP020516:c4223467-4223234 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLMVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT74963 NZ_CP020516:c4223467-4223234 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTATGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A2T1MJK4