Detailed information of TA system TA074961

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3977679..3979368
• Replicon: chromosome
• Accession: NZ_CP020516
• Organism: Escherichia coli strain 222

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A829L165
• Locus tag: A9P13_RS20655
• Protein ID: WP_001521912.1
• Coordinates: 3977679..3978599 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: A9P13_RS20660
• Protein ID: WP_001521911.1
• Coordinates: 3978586..3979368 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9P13_RS20635	3973769..3974896	-	1128	WP_001521913.1	MFS transporter	-
A9P13_RS20640	3975038..3976096	-	1059	WP_122452191.1	alpha/beta hydrolase	-
A9P13_RS20645	3976361..3977245	+	885	WP_001119037.1	LysR family transcriptional regulator	-
A9P13_RS20655	3977679..3978599	-	921	WP_001521912.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
A9P13_RS20660	3978586..3979368	-	783	WP_001521911.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
A9P13_RS20665	3979684..3982893	+	3210	WP_001521910.1	RNA-directed DNA polymerase	-
A9P13_RS20670	3983054..3983194	+	141	WP_001308408.1	ash family protein	-
A9P13_RS20675	3983240..3983710	+	471	WP_000201185.1	hypothetical protein	-
A9P13_RS28960	3983707..3983809	+	103	Protein_3785	helix-turn-helix domain-containing protein	-
A9P13_RS28965	3983901..3984059	+	159	Protein_3786	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34601.04 Da
• Isoelectric Point: 7.2649

>T74961 WP_001521912.1 NZ_CP020516:c3978599-3977679 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERITTLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

>T74961 NZ_CP020516:c3978599-3977679 [Escherichia coli]
ATGGATCTGCGTGATAGCTATGCCCGGCAGGTCAAACTGTTGATGGCCGCTCTGCCGTATGTGGCGAAGGAGTCCTGTTT
CGCGTTGAAAGGCGGCACCGCAATCAATTTGTTTGTGCAGGATTTTCCCCGTCTGTCGGTGGATATTGATCTGGCGTATA
AGAGCTTTGCGGATCGCGATACTGATCTGGCGGCTATCAACGACGCTTTGAAGAGGATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATCTGCGCAGCGCTTGATCGTCAGCAC
CCGCGCGATCTGTTTGATGTGCTGAATATGCTGGAGAAACCGGGACTGATGCGGGAGATCTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGCCCCATTGCCGAGTTGCTGGCCCCAAACTGGGATACAGAGCGGATCACGACGCTGTATGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTGCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATCTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGTAATCTGGCGGTGCTGAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGCGTAA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29168.46 Da
• Isoelectric Point: 8.4458

>AT74961 WP_001521911.1 NZ_CP020516:c3979368-3978586 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELVAYEIA
CGVPSLISFEHADALFQGMNLLSPRKLQALLCASHSVKTNRVMLYLARRNGHPYFQFIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT74961 NZ_CP020516:c3979368-3978586 [Escherichia coli]
TTGCCAAACTATCTAAACTGGCTGATGCAGCACACGCTACCCGGCCAGGTGCTGCTCCAGTCATGGCTTACCCGCAACGG
CATAGATCGTAAGCTCTCTTATCTCTATGTGAAGAATGGCTGGCTCAGGCGGATCGCTCACGGCGTATACTGCCGGACGG
GTCGTGAACCGGATTGGGTGGATGCGGTTTACTGCCTGCAATCGCAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGTCATGCGCACTATACCGAACAGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTTCTGCCTTTGAACAATCCGCAACATTTGTCACGCTGTATACGTCAGGCCTTACCGCTGATGTAA
CGCCATTTACTACGGAACTGAAAATTGGTGACCGGCAGTTAGCGGGCAGTGTCCCGGAACTGGTCGCGTATGAGATCGCT
TGTGGCGTTCCTTCGTTGATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGATGAATCTTCTCAGCCCGCGTAAATT
GCAGGCGTTACTCTGCGCCAGTCATTCGGTTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTTTATTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCCGGCGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCACGCGCATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Toxin

• AlphaFold DB: A0A829L165