Detailed information of TA system TA074959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3357612..3358317
• Replicon: chromosome
• Accession: NZ_CP020516
• Organism: Escherichia coli strain 222

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: A9P13_RS17610
• Protein ID: WP_000539521.1
• Coordinates: 3357612..3357998 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: A9P13_RS17615
• Protein ID: WP_001280945.1
• Coordinates: 3357988..3358317 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9P13_RS17590	3353616..3354242	+	627	WP_020233670.1	glutathione S-transferase GstB	-
A9P13_RS17595	3354239..3355354	-	1116	WP_000555050.1	aldose sugar dehydrogenase YliI	-
A9P13_RS17600	3355465..3355848	-	384	WP_000497137.1	biofilm formation regulator BssR	-
A9P13_RS17605	3356061..3357386	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
A9P13_RS17610	3357612..3357998	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A9P13_RS17615	3357988..3358317	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
A9P13_RS17620	3358387..3359715	-	1329	WP_000086873.1	GGDEF domain-containing protein	-
A9P13_RS17625	3359723..3362071	-	2349	WP_021523051.1	EAL domain-containing protein	-
A9P13_RS17630	3362249..3363160	-	912	WP_001236019.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T74959 WP_000539521.1 NZ_CP020516:3357612-3357998 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T74959 NZ_CP020516:3357612-3357998 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT74959 WP_001280945.1 NZ_CP020516:3357988-3358317 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT74959 NZ_CP020516:3357988-3358317 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6