Detailed information of TA system TA074949

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2678002..2678528
• Replicon: chromosome
• Accession: NZ_CP020516
• Organism: Escherichia coli strain 222

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: A9P13_RS13945
• Protein ID: WP_000323025.1
• Coordinates: 2678002..2678289 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: A9P13_RS13950
• Protein ID: WP_000534858.1
• Coordinates: 2678289..2678528 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9P13_RS13900	2673086..2673544	-	459	WP_000526135.1	IS200/IS605 family transposase	-
A9P13_RS13910	2673995..2674210	-	216	WP_000066484.1	cold shock-like protein CspB	-
A9P13_RS13915	2674511..2674723	+	213	WP_000087756.1	cold shock-like protein CspF	-
A9P13_RS28730	2674778..2674867	+	90	WP_120795389.1	hypothetical protein	-
A9P13_RS13920	2675145..2675897	-	753	WP_001047135.1	antitermination protein	-
A9P13_RS13925	2675911..2676960	-	1050	WP_001265199.1	DUF968 domain-containing protein	-
A9P13_RS13930	2676962..2677240	-	279	WP_012304870.1	hypothetical protein	-
A9P13_RS13935	2677307..2677558	-	252	WP_000980994.1	hypothetical protein	-
A9P13_RS13940	2677775..2677930	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
A9P13_RS13945	2678002..2678289	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
A9P13_RS13950	2678289..2678528	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
A9P13_RS13955	2678553..2678858	+	306	WP_001326990.1	hypothetical protein	-
A9P13_RS13960	2679061..2679393	+	333	WP_001301033.1	protein FlxA	-
A9P13_RS13970	2679830..2681143	-	1314	WP_000589005.1	ISNCY family transposase	-
A9P13_RS13975	2681627..2682655	-	1029	WP_001345551.1	DUF4868 domain-containing protein	-
A9P13_RS13980	2682652..2683266	-	615	WP_001265627.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2635257..2695753	60496
	-	flank	IS/Tn	-	-	2673086..2673544	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T74949 WP_000323025.1 NZ_CP020516:c2678289-2678002 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T74949 NZ_CP020516:c2678289-2678002 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT74949 WP_000534858.1 NZ_CP020516:c2678528-2678289 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT74949 NZ_CP020516:c2678528-2678289 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8