Detailed information of TA system TA074947

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2496840..2497478
• Replicon: chromosome
• Accession: NZ_CP020516
• Organism: Escherichia coli strain 222

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: A9P13_RS13005
• Protein ID: WP_001306887.1
• Coordinates: 2496840..2497016 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: A9P13_RS13010
• Protein ID: WP_001270286.1
• Coordinates: 2497062..2497478 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9P13_RS12980	2491896..2492354	+	459	WP_000526135.1	IS200/IS605 family transposase	-
A9P13_RS12985	2492462..2493673	-	1212	WP_023143671.1	BenE family transporter YdcO	-
A9P13_RS12990	2493726..2494262	+	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
A9P13_RS12995	2494335..2496296	+	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
A9P13_RS13000	2496388..2496618	-	231	WP_000494241.1	YncJ family protein	-
A9P13_RS13005	2496840..2497016	+	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
A9P13_RS13010	2497062..2497478	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
A9P13_RS13015	2497557..2498963	+	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
A9P13_RS13020	2499208..2500353	+	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
A9P13_RS13025	2500371..2501384	+	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
A9P13_RS13030	2501385..2502326	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2491896..2492354	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T74947 WP_001306887.1 NZ_CP020516:2496840-2497016 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T74947 NZ_CP020516:2496840-2497016 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCGTGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT74947 WP_001270286.1 NZ_CP020516:2497062-2497478 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT74947 NZ_CP020516:2497062-2497478 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76