Detailed information of TA system TA074933

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 101095..101654
• Replicon: plasmid unnamed
• Accession: NZ_CP020515
• Organism: Escherichia coli strain 219

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0SVC8
• Locus tag: A9P12_RS26385
• Protein ID: WP_000421272.1
• Coordinates: 101095..101370 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SRR3
• Locus tag: A9P12_RS26390
• Protein ID: WP_001387467.1
• Coordinates: 101370..101654 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9P12_RS26345	97145..97885	-	741	WP_001066942.1	tyrosine-type recombinase/integrase	-
A9P12_RS26350	98006..98188	-	183	WP_042065278.1	hypothetical protein	-
A9P12_RS26360	98531..98838	-	308	Protein_111	type II toxin-antitoxin system ParD family antitoxin	-
A9P12_RS26370	99331..100500	+	1170	WP_001072355.1	hypothetical protein	-
A9P12_RS26375	100533..100661	+	129	Protein_113	IS481 family transposase	-
A9P12_RS26380	100696..100989	-	294	WP_001105060.1	hypothetical protein	-
A9P12_RS26385	101095..101370	-	276	WP_000421272.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A9P12_RS26390	101370..101654	-	285	WP_001387467.1	ribbon-helix-helix domain-containing protein	Antitoxin
A9P12_RS26395	102259..103011	+	753	WP_000562172.1	IclR family transcriptional regulator	-
A9P12_RS26400	103057..104022	-	966	WP_001022265.1	2-keto-3-deoxygluconate transporter	-
A9P12_RS26405	104055..104435	-	381	WP_000710783.1	Rid family detoxifying hydrolase	-
A9P12_RS26410	104460..105350	-	891	WP_001077068.1	dihydrodipicolinate synthase family protein	-
A9P12_RS26415	105583..105777	-	195	WP_000796505.1	hypothetical protein	-
A9P12_RS26420	105751..106191	+	441	Protein_122	zinc-binding dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr	-	1..158757	158757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 10.0871

>T74933 WP_000421272.1 NZ_CP020515:c101370-101095 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T74933 NZ_CP020515:c101370-101095 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT74933 WP_001387467.1 NZ_CP020515:c101654-101370 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT74933 NZ_CP020515:c101654-101370 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: V0SVC8

Antitoxin

• AlphaFold DB: A0A0E0Y880