74931

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 83194..83719 Replicon plasmid unnamed
Accession NZ_CP020515
Organism Escherichia coli strain 219

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag A9P12_RS26265 Protein ID WP_001159868.1
Coordinates 83194..83499 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag A9P12_RS26270 Protein ID WP_000813634.1
Coordinates 83501..83719 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9P12_RS26250 79104..80270 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
A9P12_RS26255 80858..81613 - 756 WP_000852146.1 replication initiation protein RepE -
A9P12_RS26260 82387..83193 - 807 WP_000016982.1 site-specific integrase -
A9P12_RS26265 83194..83499 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
A9P12_RS26270 83501..83719 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
A9P12_RS26275 84353..84550 + 198 WP_000215657.1 hypothetical protein -
A9P12_RS26280 84547..84831 - 285 WP_000642771.1 hypothetical protein -
A9P12_RS26285 84851..85984 - 1134 WP_000545983.1 DUF3800 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr - 1..158757 158757


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T74931 WP_001159868.1 NZ_CP020515:c83499-83194 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T74931 NZ_CP020515:c83499-83194 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT74931 WP_000813634.1 NZ_CP020515:c83719-83501 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT74931 NZ_CP020515:c83719-83501 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References