Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 973627..974281 Replicon chromosome
Accession NZ_CP020514
Organism Escherichia coli strain 219

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag A9P12_RS05205 Protein ID WP_000244777.1
Coordinates 973874..974281 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag A9P12_RS05200 Protein ID WP_000354046.1
Coordinates 973627..973893 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9P12_RS05175 968796..969539 + 744 WP_000951964.1 SDR family oxidoreductase -
A9P12_RS05180 969596..971029 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
A9P12_RS05185 971074..971385 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
A9P12_RS05190 971549..972208 + 660 WP_000250274.1 hemolysin III family protein -
A9P12_RS05195 972404..973384 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
A9P12_RS05200 973627..973893 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
A9P12_RS05205 973874..974281 + 408 WP_000244777.1 toxin CptA Toxin
A9P12_RS05210 974321..974842 - 522 WP_001055874.1 flavodoxin FldB -
A9P12_RS05215 974954..975850 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
A9P12_RS05220 975875..976585 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
A9P12_RS05225 976591..978324 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T74904 WP_000244777.1 NZ_CP020514:973874-974281 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T74904 NZ_CP020514:973874-974281 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT74904 WP_000354046.1 NZ_CP020514:973627-973893 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT74904 NZ_CP020514:973627-973893 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References