Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 38902..39427 Replicon plasmid unnamed1
Accession NZ_CP020510
Organism Escherichia coli strain 165

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag AWB10_RS26820 Protein ID WP_001159871.1
Coordinates 39122..39427 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag AWB10_RS26815 Protein ID WP_000813634.1
Coordinates 38902..39120 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWB10_RS26780 34773..35402 + 630 Protein_38 hypothetical protein -
AWB10_RS26785 35561..36301 - 741 WP_072652231.1 abortive infection family protein -
AWB10_RS26790 36337..37125 - 789 WP_072652230.1 DUF2971 domain-containing protein -
AWB10_RS26795 37122..37742 - 621 WP_072652229.1 hypothetical protein -
AWB10_RS26800 37739..38434 - 696 WP_072652234.1 DNA-binding protein -
AWB10_RS26815 38902..39120 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AWB10_RS26820 39122..39427 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
AWB10_RS26825 39428..40237 + 810 WP_000016963.1 site-specific integrase -
AWB10_RS26830 40409..40537 + 129 Protein_46 colicin M immunity protein -
AWB10_RS26835 40589..41286 + 698 Protein_47 IS1 family transposase -
AWB10_RS26840 41297..41835 + 539 Protein_48 GGDEF domain-containing protein -
AWB10_RS26845 41930..42601 + 672 WP_046788564.1 FadR family transcriptional regulator -
AWB10_RS26850 42660..43685 - 1026 WP_000864945.1 zinc-binding alcohol dehydrogenase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / aac(3)-IId / rmtB / sul1 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / tet(A) - 1..118182 118182


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T74889 WP_001159871.1 NZ_CP020510:39122-39427 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T74889 NZ_CP020510:39122-39427 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT74889 WP_000813634.1 NZ_CP020510:38902-39120 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT74889 NZ_CP020510:38902-39120 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References