Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3657078..3657696 Replicon chromosome
Accession NZ_CP020509
Organism Escherichia coli strain 165

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AWB10_RS19070 Protein ID WP_001291435.1
Coordinates 3657478..3657696 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AWB10_RS19065 Protein ID WP_000344800.1
Coordinates 3657078..3657452 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWB10_RS19055 3652167..3653360 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AWB10_RS19060 3653383..3656532 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
AWB10_RS19065 3657078..3657452 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AWB10_RS19070 3657478..3657696 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
AWB10_RS19075 3657867..3658418 + 552 WP_000102564.1 maltose O-acetyltransferase -
AWB10_RS19080 3658534..3659004 + 471 WP_000136192.1 YlaC family protein -
AWB10_RS19085 3659168..3660718 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AWB10_RS19090 3660760..3661113 - 354 WP_000878141.1 DUF1428 family protein -
AWB10_RS19100 3661492..3661803 + 312 WP_000409911.1 MGMT family protein -
AWB10_RS19105 3661834..3662406 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T74883 WP_001291435.1 NZ_CP020509:3657478-3657696 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T74883 NZ_CP020509:3657478-3657696 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT74883 WP_000344800.1 NZ_CP020509:3657078-3657452 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT74883 NZ_CP020509:3657078-3657452 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References