Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 718174..719015 Replicon chromosome
Accession NZ_CP020509
Organism Escherichia coli strain 165

Toxin (Protein)


Gene name yeeV Uniprot ID A0A1M0WJM1
Locus tag AWB10_RS03725 Protein ID WP_000854822.1
Coordinates 718632..719015 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A243ZR83
Locus tag AWB10_RS03720 Protein ID WP_071587515.1
Coordinates 718174..718542 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWB10_RS03700 715918..716736 + 819 WP_001234702.1 DUF945 domain-containing protein -
AWB10_RS03705 716827..717312 + 486 WP_063097556.1 antirestriction protein -
AWB10_RS03710 717328..717804 + 477 WP_001186740.1 RadC family protein -
AWB10_RS03715 717873..718094 + 222 WP_000692312.1 DUF987 domain-containing protein -
AWB10_RS03720 718174..718542 + 369 WP_071587515.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
AWB10_RS03725 718632..719015 + 384 WP_000854822.1 type IV toxin-antitoxin system toxin CbtA Toxin
AWB10_RS03730 719006..719494 + 489 WP_001177592.1 hypothetical protein -
AWB10_RS03735 719506..719703 + 198 WP_000839263.1 DUF957 domain-containing protein -
AWB10_RS03740 719800..720645 + 846 WP_072735927.1 DUF4942 domain-containing protein -
AWB10_RS03750 720944..721450 + 507 WP_000228937.1 G/U mismatch-specific DNA glycosylase -
AWB10_RS03755 721528..723369 - 1842 WP_000437371.1 RNA polymerase sigma factor RpoD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14295.32 Da        Isoelectric Point: 8.2830

>T74866 WP_000854822.1 NZ_CP020509:718632-719015 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHQESKRCN

Download         Length: 384 bp

>T74866 NZ_CP020509:718632-719015 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCAGGAGTCGAAACGATGCAACTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13631.41 Da        Isoelectric Point: 6.6244

>AT74866 WP_071587515.1 NZ_CP020509:718174-718542 [Escherichia coli]
VSDTLPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLTDRAGIRGRFSDADANHLDQAFPLLMKQLELMLTSS
ELNPHRQNTVTLYVKGLTCHADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT74866 NZ_CP020509:718174-718542 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTACAGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGATGCGGATGCGAACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCAGT
GAACTGAATCCCCACCGTCAGAATACCGTCACGCTGTATGTTAAAGGGCTGACCTGCCATGCGGACACCCTCGGCAGCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M0WJM1

Antitoxin

Source ID Structure
AlphaFold DB A0A243ZR83

References