74863

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 179446..179972 Replicon plasmid unnamed
Accession NZ_CP020508
Organism Serratia marcescens strain BWH-35

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag AS658_RS26475 Protein ID WP_000323025.1
Coordinates 179685..179972 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q5J3S4
Locus tag AS658_RS26470 Protein ID WP_000534857.1
Coordinates 179446..179685 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AS658_RS26455 176079..176684 + 606 WP_000509966.1 recombinase family protein -
AS658_RS26460 177079..178239 - 1161 WP_075335826.1 IS30 family transposase -
AS658_RS26465 178449..179189 - 741 WP_022652289.1 hypothetical protein -
AS658_RS26470 179446..179685 + 240 WP_000534857.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
AS658_RS26475 179685..179972 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
AS658_RS26480 180255..180479 + 225 Protein_188 hypothetical protein -
AS658_RS26490 180774..181790 + 1017 WP_086528432.1 IS5-like element IS5 family transposase -
AS658_RS26495 181931..182125 + 195 Protein_190 endonuclease -
AS658_RS26500 182304..182936 + 633 WP_022652286.1 recombinase family protein -
AS658_RS26505 182990..183190 + 201 WP_040219232.1 hypothetical protein -
AS658_RS26510 183337..184287 + 951 WP_022652284.1 Rpn family recombination-promoting nuclease/putative transposase -
AS658_RS26515 184284..184895 - 612 WP_022652283.1 DUF2913 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..224208 224208
- inside IScluster/Tn - - 172375..204062 31687


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T74863 WP_000323025.1 NZ_CP020508:179685-179972 [Serratia marcescens]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T74863 NZ_CP020508:179685-179972 [Serratia marcescens]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9085.55 Da        Isoelectric Point: 4.6820

>AT74863 WP_000534857.1 NZ_CP020508:179446-179685 [Serratia marcescens]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRKPKPVRVTLDEL

Download         Length: 240 bp

>AT74863 NZ_CP020508:179446-179685 [Serratia marcescens]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAAGCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE60

References