74862

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 140523..141326 Replicon plasmid unnamed
Accession NZ_CP020508
Organism Serratia marcescens strain BWH-35

Toxin (Protein)


Gene name KacT Uniprot ID A0A7W3E3C9
Locus tag AS658_RS26285 Protein ID WP_022652313.1
Coordinates 140523..141053 (-) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag AS658_RS26290 Protein ID WP_022652312.1
Coordinates 141057..141326 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AS658_RS26250 136409..136756 - 348 WP_000609174.1 IS66 family insertion sequence element accessory protein TnpB -
AS658_RS26255 136753..137136 - 384 WP_001201739.1 IS66 family insertion sequence hypothetical protein -
AS658_RS26260 137245..138396 - 1152 WP_022652315.1 IS30-like element IS30 family transposase -
AS658_RS26270 138461..139357 + 897 Protein_149 IS3 family transposase -
AS658_RS26275 139498..140391 - 894 WP_077873527.1 IS5 family transposase -
AS658_RS26285 140523..141053 - 531 WP_022652313.1 GNAT family N-acetyltransferase Toxin
AS658_RS26290 141057..141326 - 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
AS658_RS26295 142182..143171 + 990 WP_022652311.1 RepB family plasmid replication initiator protein -
AS658_RS26300 143497..144237 - 741 WP_022652310.1 site-specific integrase -
AS658_RS26310 144714..145021 + 308 Protein_155 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..224208 224208
- inside IScluster/Tn - - 132954..146335 13381


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20349.26 Da        Isoelectric Point: 6.7496

>T74862 WP_022652313.1 NZ_CP020508:c141053-140523 [Serratia marcescens]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

>T74862 NZ_CP020508:c141053-140523 [Serratia marcescens]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGA
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT74862 WP_022652312.1 NZ_CP020508:c141326-141057 [Serratia marcescens]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT74862 NZ_CP020508:c141326-141057 [Serratia marcescens]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3E3C9

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References