74861

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 102898..103541 Replicon plasmid unnamed
Accession NZ_CP020508
Organism Serratia marcescens strain BWH-35

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag AS658_RS26100 Protein ID WP_001044770.1
Coordinates 103125..103541 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag AS658_RS26095 Protein ID WP_001261282.1
Coordinates 102898..103128 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AS658_RS26065 97924..98892 - 969 WP_077254959.1 IS5 family transposase -
AS658_RS26070 99064..99846 - 783 WP_048235058.1 site-specific integrase -
AS658_RS26075 99884..100231 - 348 WP_074405026.1 hypothetical protein -
AS658_RS26080 100593..101402 - 810 WP_053059919.1 hypothetical protein -
AS658_RS26085 101399..102319 - 921 WP_048235060.1 hypothetical protein -
AS658_RS27085 102480..102941 - 462 WP_195765657.1 hypothetical protein -
AS658_RS26095 102898..103128 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AS658_RS26100 103125..103541 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
AS658_RS26105 103615..105177 + 1563 WP_004206609.1 AAA family ATPase -
AS658_RS26110 105162..106184 + 1023 WP_000361404.1 helicase UvrD -
AS658_RS26115 106722..107636 + 915 WP_031592169.1 HNH endonuclease -
AS658_RS26120 107822..108172 - 351 WP_004187110.1 DUF305 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..224208 224208
- flank IS/Tn - - 97924..98892 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T74861 WP_001044770.1 NZ_CP020508:103125-103541 [Serratia marcescens]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T74861 NZ_CP020508:103125-103541 [Serratia marcescens]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT74861 WP_001261282.1 NZ_CP020508:102898-103128 [Serratia marcescens]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT74861 NZ_CP020508:102898-103128 [Serratia marcescens]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References