74860

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2634..3370 Replicon plasmid unnamed
Accession NZ_CP020508
Organism Serratia marcescens strain BWH-35

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag AS658_RS25490 Protein ID WP_003026803.1
Coordinates 2888..3370 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AS658_RS25485 Protein ID WP_003026799.1
Coordinates 2634..2900 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AS658_RS25455 85..651 + 567 WP_009310052.1 hypothetical protein -
AS658_RS25460 682..1176 + 495 WP_009310053.1 hypothetical protein -
AS658_RS25465 1220..1588 + 369 WP_009310054.1 hypothetical protein -
AS658_RS25470 1619..1822 + 204 WP_004098931.1 hemolysin expression modulator Hha -
AS658_RS25475 1871..2128 + 258 WP_004098928.1 hypothetical protein -
AS658_RS25480 2204..2458 + 255 WP_004118702.1 hypothetical protein -
AS658_RS25485 2634..2900 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AS658_RS25490 2888..3370 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
AS658_RS25495 3571..4974 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
AS658_RS25500 5003..5635 - 633 WP_001567369.1 hypothetical protein -
AS658_RS25505 5854..7257 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
AS658_RS25510 7286..7918 - 633 WP_001567369.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..224208 224208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T74860 WP_003026803.1 NZ_CP020508:2888-3370 [Serratia marcescens]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T74860 NZ_CP020508:2888-3370 [Serratia marcescens]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT74860 WP_003026799.1 NZ_CP020508:2634-2900 [Serratia marcescens]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT74860 NZ_CP020508:2634-2900 [Serratia marcescens]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References