Detailed information of TA system TA074841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 15055..15656
• Replicon: chromosome
• Accession: NZ_CP020507
• Organism: Serratia marcescens strain BWH-35

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AS658_RS00090
• Protein ID: WP_038876000.1
• Coordinates: 15055..15435 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0G3SPC0
• Locus tag: AS658_RS00095
• Protein ID: WP_038875999.1
• Coordinates: 15435..15656 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AS658_RS00055	10843..11190	-	348	WP_016929593.1	YceK/YidQ family lipoprotein	-
AS658_RS00060	11500..11790	+	291	WP_080347946.1	Hsp20 family protein	-
AS658_RS00070	11841..12538	+	698	Protein_11	IS1 family transposase	-
AS658_RS00075	12555..12689	+	135	Protein_12	Hsp20 family protein	-
AS658_RS00080	12790..13218	+	429	WP_004933922.1	heat shock chaperone IbpB	-
AS658_RS00085	13386..15044	+	1659	WP_047729572.1	putative transporter	-
AS658_RS00090	15055..15435	-	381	WP_038876000.1	type II toxin-antitoxin system VapC family toxin	Toxin
AS658_RS00095	15435..15656	-	222	WP_038875999.1	ribbon-helix-helix domain-containing protein	Antitoxin
AS658_RS00100	15731..16981	-	1251	WP_038875998.1	valine--pyruvate transaminase	-
AS658_RS00105	17117..19180	-	2064	WP_048232293.1	alpha-amylase	-
AS658_RS26960	19376..19528	+	153	WP_171887318.1	hypothetical protein	-
AS658_RS00110	19530..20507	-	978	WP_048232294.1	glyoxylate/hydroxypyruvate reductase GhrB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	12035..12538	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-102)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14167.50 Da
• Isoelectric Point: 7.3170

>T74841 WP_038876000.1 NZ_CP020507:c15435-15055 [Serratia marcescens]
MVAQRALFDTNILIDYLNGIPQAKDVLTEYHINPAISAITWMEVIVGAKKQGPALELKTRQFLGQFLLLPITDEVAERAV
ELRHSQHVKLPDAIIWATAQVGFRTLISRNPKDFGTDNGVLMPYRL

Nucleotide

• Length: 381 bp

>T74841 NZ_CP020507:c15435-15055 [Serratia marcescens]
ATGGTGGCTCAACGGGCGTTATTCGACACCAATATCCTGATCGATTATCTCAACGGCATTCCTCAAGCCAAAGACGTTCT
GACCGAATACCACATCAACCCGGCTATTAGCGCCATCACCTGGATGGAAGTGATAGTGGGTGCCAAAAAACAGGGGCCGG
CGCTGGAGCTGAAAACGCGCCAGTTTTTAGGGCAATTCCTGCTGCTGCCTATTACCGATGAAGTTGCCGAACGCGCCGTA
GAATTGCGCCACTCACAGCATGTGAAGCTGCCGGACGCCATTATCTGGGCCACCGCGCAGGTGGGGTTCCGGACGCTCAT
CTCCCGCAACCCCAAAGATTTTGGCACTGACAACGGCGTGTTAATGCCTTACCGGCTGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8563.50 Da
• Isoelectric Point: 4.4791

>AT74841 WP_038875999.1 NZ_CP020507:c15656-15435 [Serratia marcescens]
MTRIVIDLPEEDLRQLDNLKNIRHVPRAEIIRQAVSRYLAENHVEDPSNAFGLWSGAMGDGVDYENRQREEWE

Nucleotide

• Length: 222 bp

>AT74841 NZ_CP020507:c15656-15435 [Serratia marcescens]
ATGACTCGCATCGTGATCGATTTACCTGAAGAAGACCTGCGGCAGCTCGACAATCTGAAAAATATCCGCCACGTTCCCCG
GGCCGAAATCATCCGTCAGGCCGTCTCCCGTTATCTGGCGGAGAACCACGTTGAAGATCCCAGCAATGCCTTCGGGCTCT
GGTCCGGGGCGATGGGCGATGGCGTAGACTACGAAAATCGTCAGCGCGAGGAGTGGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0G3SPC0