Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 183568..184304 Replicon plasmid unnamed1
Accession NZ_CP020506
Organism Serratia marcescens strain 95

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag AM681_RS27245 Protein ID WP_003026803.1
Coordinates 183568..184050 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AM681_RS27250 Protein ID WP_003026799.1
Coordinates 184038..184304 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM681_RS27225 179287..179682 - 396 WP_021312679.1 helix-turn-helix domain-containing protein -
AM681_RS27230 179731..181077 - 1347 WP_077259381.1 ISNCY family transposase -
AM681_RS27235 181303..181935 + 633 WP_001567369.1 hypothetical protein -
AM681_RS27240 181964..183367 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
AM681_RS27245 183568..184050 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
AM681_RS27250 184038..184304 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AM681_RS27255 184480..184734 - 255 WP_004118702.1 hypothetical protein -
AM681_RS27260 184810..185067 - 258 WP_004098928.1 hypothetical protein -
AM681_RS27265 185116..185319 - 204 WP_004098931.1 hemolysin expression modulator Hha -
AM681_RS27270 185350..185718 - 369 WP_009310054.1 hypothetical protein -
AM681_RS27275 185762..186256 - 495 WP_009310053.1 hypothetical protein -
AM681_RS27280 186287..186853 - 567 WP_009310052.1 hypothetical protein -
AM681_RS27285 186850..187113 - 264 WP_009310051.1 hypothetical protein -
AM681_RS27290 187427..187606 + 180 WP_004118349.1 type II toxin-antitoxin system ParD family antitoxin -
AM681_RS27295 187593..187691 + 99 WP_004118688.1 type II toxin-antitoxin system RelE/ParE family toxin -
AM681_RS27305 188070..188504 - 435 WP_004118347.1 hypothetical protein -
AM681_RS27310 188720..188866 - 147 Protein_202 two-component sensor histidine kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..219979 219979


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T74840 WP_003026803.1 NZ_CP020506:c184050-183568 [Serratia marcescens]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T74840 NZ_CP020506:c184050-183568 [Serratia marcescens]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT74840 WP_003026799.1 NZ_CP020506:c184304-184038 [Serratia marcescens]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT74840 NZ_CP020506:c184304-184038 [Serratia marcescens]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References