Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 85681..86324 Replicon plasmid unnamed1
Accession NZ_CP020506
Organism Serratia marcescens strain 95

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag AM681_RS26645 Protein ID WP_001044770.1
Coordinates 85681..86097 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag AM681_RS26650 Protein ID WP_001261282.1
Coordinates 86094..86324 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM681_RS26625 81050..81400 + 351 WP_004187110.1 DUF305 domain-containing protein -
AM681_RS26630 81586..82500 - 915 WP_031592169.1 HNH endonuclease -
AM681_RS26635 83038..84060 - 1023 WP_000361404.1 helicase UvrD -
AM681_RS26640 84045..85607 - 1563 WP_004206609.1 AAA family ATPase -
AM681_RS26645 85681..86097 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
AM681_RS26650 86094..86324 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AM681_RS27845 86281..86742 + 462 WP_195765657.1 hypothetical protein -
AM681_RS26660 86903..87823 + 921 WP_048235060.1 hypothetical protein -
AM681_RS26665 87820..88629 + 810 WP_053059919.1 hypothetical protein -
AM681_RS26670 88991..89338 + 348 WP_074405026.1 hypothetical protein -
AM681_RS26675 89376..90158 + 783 WP_048235058.1 site-specific integrase -
AM681_RS26680 90330..91298 + 969 WP_077254959.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..219979 219979
- flank IS/Tn - - 90330..91298 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T74839 WP_001044770.1 NZ_CP020506:c86097-85681 [Serratia marcescens]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T74839 NZ_CP020506:c86097-85681 [Serratia marcescens]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT74839 WP_001261282.1 NZ_CP020506:c86324-86094 [Serratia marcescens]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT74839 NZ_CP020506:c86324-86094 [Serratia marcescens]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References