Detailed information of TA system TA074832

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3218993..3219624
• Replicon: chromosome
• Accession: NZ_CP020503
• Organism: Serratia marcescens strain 95

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AM681_RS15760
• Protein ID: WP_048234362.1
• Coordinates: 3218993..3219391 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0G3SVC2
• Locus tag: AM681_RS15765
• Protein ID: WP_047730457.1
• Coordinates: 3219391..3219624 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM681_RS15740	3214096..3214911	-	816	WP_047730461.1	5'-nucleotidase, lipoprotein e(P4) family	-
AM681_RS15745	3215240..3215644	-	405	WP_047730460.1	flagellar protein FlhE	-
AM681_RS15750	3215644..3217722	-	2079	WP_038876828.1	flagellar biosynthesis protein FlhA	-
AM681_RS15755	3217715..3218866	-	1152	WP_047730459.1	flagellar type III secretion system protein FlhB	-
AM681_RS15760	3218993..3219391	-	399	WP_048234362.1	tRNA(fMet)-specific endonuclease VapC	Toxin
AM681_RS15765	3219391..3219624	-	234	WP_047730457.1	antitoxin	Antitoxin
AM681_RS15770	3219750..3220394	-	645	WP_004934858.1	protein phosphatase CheZ	-
AM681_RS15775	3220405..3220794	-	390	WP_004934862.1	chemotaxis response regulator CheY	-
AM681_RS15780	3220897..3221946	-	1050	WP_015378262.1	chemotaxis response regulator protein-glutamate methylesterase	-
AM681_RS15785	3221946..3222818	-	873	WP_025159837.1	protein-glutamate O-methyltransferase CheR	-
AM681_RS15790	3222856..3224472	-	1617	WP_048234363.1	Tar ligand binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-120)

Antitoxin

• SpoVT-AbrB domain (7-45)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14907.09 Da
• Isoelectric Point: 6.9789

>T74832 WP_048234362.1 NZ_CP020503:c3219391-3218993 [Serratia marcescens]
MFSHMLDTNIVIYVIKRRPLEVLEVFNRYAGKMVISSITYGELVHGVENSARPAVNARVVEDFVSRLDILDYSAKAASHY
GNIRAVLERQGTPIGVNDLHIAGHARSEGLILVTNNRREFECVEGLRLENWL

Nucleotide

• Length: 399 bp

>T74832 NZ_CP020503:c3219391-3218993 [Serratia marcescens]
ATGTTCAGCCATATGCTGGACACCAACATTGTTATCTATGTCATTAAGCGCCGGCCGCTGGAAGTGCTGGAGGTATTCAA
TCGCTATGCAGGTAAAATGGTCATTTCGTCGATTACCTATGGCGAATTGGTTCACGGGGTGGAGAACAGCGCTCGCCCAG
CGGTGAACGCCAGAGTCGTTGAGGATTTTGTCTCCCGATTGGACATTCTCGATTACAGCGCCAAAGCGGCATCGCACTAC
GGCAACATCCGGGCTGTGCTGGAACGCCAGGGAACGCCTATTGGCGTAAACGATCTACATATCGCCGGGCATGCGCGCAG
CGAAGGATTGATCCTGGTGACCAATAATCGACGCGAATTTGAGTGCGTTGAGGGTTTGCGGCTGGAAAATTGGTTGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8841.85 Da
• Isoelectric Point: 4.7815

>AT74832 WP_047730457.1 NZ_CP020503:c3219624-3219391 [Serratia marcescens]
MALGSVFTNNRTQAVRLPAEVRFPDTVSKVTVRVVGKERILAPLENTWDSFFCAENEVSDDFLNERAGQHQREREDF

Nucleotide

• Length: 234 bp

>AT74832 NZ_CP020503:c3219624-3219391 [Serratia marcescens]
ATGGCGTTAGGTTCTGTCTTTACCAATAATCGGACACAGGCTGTACGCCTGCCTGCAGAGGTGCGTTTCCCTGATACCGT
TTCTAAAGTGACCGTCCGCGTAGTGGGCAAAGAGCGCATTCTTGCCCCCCTGGAGAATACCTGGGACAGCTTCTTCTGTG
CGGAAAATGAGGTGAGCGATGATTTCCTCAATGAACGTGCCGGACAGCACCAACGCGAGCGGGAAGATTTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0G3SVC2