Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 15055..15656 Replicon chromosome
Accession NZ_CP020503
Organism Serratia marcescens strain 95

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag AM681_RS00090 Protein ID WP_038876000.1
Coordinates 15055..15435 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0G3SPC0
Locus tag AM681_RS00095 Protein ID WP_038875999.1
Coordinates 15435..15656 (-) Length 74 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM681_RS00055 10843..11190 - 348 WP_016929593.1 YceK/YidQ family lipoprotein -
AM681_RS00060 11500..11790 + 291 WP_080347946.1 Hsp20 family protein -
AM681_RS00070 11841..12538 + 698 Protein_11 IS1 family transposase -
AM681_RS00075 12555..12689 + 135 Protein_12 Hsp20 family protein -
AM681_RS00080 12790..13218 + 429 WP_004933922.1 heat shock chaperone IbpB -
AM681_RS00085 13386..15044 + 1659 WP_047729572.1 putative transporter -
AM681_RS00090 15055..15435 - 381 WP_038876000.1 type II toxin-antitoxin system VapC family toxin Toxin
AM681_RS00095 15435..15656 - 222 WP_038875999.1 ribbon-helix-helix domain-containing protein Antitoxin
AM681_RS00100 15731..16981 - 1251 WP_038875998.1 valine--pyruvate transaminase -
AM681_RS00105 17117..19180 - 2064 WP_048232293.1 alpha-amylase -
AM681_RS27710 19376..19528 + 153 WP_171887318.1 hypothetical protein -
AM681_RS00110 19530..20507 - 978 WP_048232294.1 glyoxylate/hydroxypyruvate reductase GhrB -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 12035..12538 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(7-102)

Antitoxin

(3-40)


Sequences


Toxin        


Download         Length: 127 a.a.        Molecular weight: 14167.50 Da        Isoelectric Point: 7.3170

>T74818 WP_038876000.1 NZ_CP020503:c15435-15055 [Serratia marcescens]
MVAQRALFDTNILIDYLNGIPQAKDVLTEYHINPAISAITWMEVIVGAKKQGPALELKTRQFLGQFLLLPITDEVAERAV
ELRHSQHVKLPDAIIWATAQVGFRTLISRNPKDFGTDNGVLMPYRL

Download         Length: 381 bp

>T74818 NZ_CP020503:c15435-15055 [Serratia marcescens]
ATGGTGGCTCAACGGGCGTTATTCGACACCAATATCCTGATCGATTATCTCAACGGCATTCCTCAAGCCAAAGACGTTCT
GACCGAATACCACATCAACCCGGCTATTAGCGCCATCACCTGGATGGAAGTGATAGTGGGTGCCAAAAAACAGGGGCCGG
CGCTGGAGCTGAAAACGCGCCAGTTTTTAGGGCAATTCCTGCTGCTGCCTATTACCGATGAAGTTGCCGAACGCGCCGTA
GAATTGCGCCACTCACAGCATGTGAAGCTGCCGGACGCCATTATCTGGGCCACCGCGCAGGTGGGGTTCCGGACGCTCAT
CTCCCGCAACCCCAAAGATTTTGGCACTGACAACGGCGTGTTAATGCCTTACCGGCTGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8563.50 Da        Isoelectric Point: 4.4791

>AT74818 WP_038875999.1 NZ_CP020503:c15656-15435 [Serratia marcescens]
MTRIVIDLPEEDLRQLDNLKNIRHVPRAEIIRQAVSRYLAENHVEDPSNAFGLWSGAMGDGVDYENRQREEWE

Download         Length: 222 bp

>AT74818 NZ_CP020503:c15656-15435 [Serratia marcescens]
ATGACTCGCATCGTGATCGATTTACCTGAAGAAGACCTGCGGCAGCTCGACAATCTGAAAAATATCCGCCACGTTCCCCG
GGCCGAAATCATCCGTCAGGCCGTCTCCCGTTATCTGGCGGAGAACCACGTTGAAGATCCCAGCAATGCCTTCGGGCTCT
GGTCCGGGGCGATGGGCGATGGCGTAGACTACGAAAATCGTCAGCGCGAGGAGTGGGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A0G3SPC0

References