Detailed information of TA system TA074817

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 22192..22835
• Replicon: plasmid unnamed2
• Accession: NZ_CP020500
• Organism: Klebsiella pneumoniae strain BWHC1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: AA595_RS27910
• Protein ID: WP_001044770.1
• Coordinates: 22419..22835 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: AA595_RS27905
• Protein ID: WP_001261282.1
• Coordinates: 22192..22422 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AA595_RS27885	19713..20489	-	777	WP_000015958.1	site-specific integrase	-
AA595_RS27890	20486..21229	-	744	WP_000129823.1	hypothetical protein	-
AA595_RS27895	21280..21630	-	351	WP_000493378.1	hypothetical protein	-
AA595_RS28915	21774..22235	-	462	WP_072202616.1	hypothetical protein	-
AA595_RS27905	22192..22422	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AA595_RS27910	22419..22835	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
AA595_RS27915	22909..23619	+	711	WP_004152334.1	ATP-binding protein	-
AA595_RS27920	23653..24350	+	698	Protein_27	IS1 family transposase	-
AA595_RS27925	24366..24476	+	111	Protein_28	mercuric transport protein periplasmic component	-
AA595_RS27930	24512..24934	+	423	WP_001340589.1	organomercurial transporter MerC	-
AA595_RS27935	24986..26680	+	1695	WP_000105636.1	mercury(II) reductase	-
AA595_RS27940	26698..27060	+	363	WP_001277456.1	mercury resistance co-regulator MerD	-
AA595_RS27945	27057..27293	+	237	WP_000993386.1	broad-spectrum mercury transporter MerE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aac(6')-Ib / blaTEM-1A / blaOXA-9 / blaKPC-2 / aph(4)-Ia / aac(3)-IVa / sul3 / ant(3'')-Ia / cmlA1 / aadA2	-	1..73467	73467
	-	inside	IScluster/Tn	aac(6')-Ib	-	13874..24350	10476

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T74817 WP_001044770.1 NZ_CP020500:22419-22835 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T74817 NZ_CP020500:22419-22835 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT74817 WP_001261282.1 NZ_CP020500:22192-22422 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT74817 NZ_CP020500:22192-22422 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3