Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3780011..3780629 Replicon chromosome
Accession NZ_CP020495
Organism Escherichia coli strain 103

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CPA47_RS18750 Protein ID WP_001291435.1
Coordinates 3780411..3780629 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CPA47_RS18745 Protein ID WP_000344800.1
Coordinates 3780011..3780385 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPA47_RS18735 3775100..3776293 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CPA47_RS18740 3776316..3779465 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
CPA47_RS18745 3780011..3780385 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CPA47_RS18750 3780411..3780629 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CPA47_RS18755 3780801..3781352 + 552 WP_001522034.1 maltose O-acetyltransferase -
CPA47_RS18760 3781468..3781938 + 471 WP_001490888.1 YlaC family protein -
CPA47_RS18765 3782102..3783652 + 1551 WP_001577872.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CPA47_RS18770 3783694..3784047 - 354 WP_000878140.1 DUF1428 domain-containing protein -
CPA47_RS18780 3784426..3784737 + 312 WP_000409911.1 MGMT family protein -
CPA47_RS18785 3784768..3785340 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T74776 WP_001291435.1 NZ_CP020495:3780411-3780629 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T74776 NZ_CP020495:3780411-3780629 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT74776 WP_000344800.1 NZ_CP020495:3780011-3780385 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT74776 NZ_CP020495:3780011-3780385 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References