Detailed information of TA system TA074765

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2678001..2678527
• Replicon: chromosome
• Accession: NZ_CP020495
• Organism: Escherichia coli strain 103

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: CPA47_RS13325
• Protein ID: WP_000323025.1
• Coordinates: 2678001..2678288 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: CPA47_RS13330
• Protein ID: WP_000534858.1
• Coordinates: 2678288..2678527 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPA47_RS13275	2673085..2673543	-	459	WP_000526135.1	IS200/IS605 family transposase	-
CPA47_RS13285	2673994..2674209	-	216	WP_000066484.1	cold shock-like protein CspB	-
CPA47_RS13290	2674510..2674722	+	213	WP_000087756.1	cold shock-like protein CspF	-
CPA47_RS13295	2674777..2674866	+	90	WP_120795389.1	hypothetical protein	-
CPA47_RS13300	2675144..2675896	-	753	WP_001047135.1	antitermination protein	-
CPA47_RS13305	2675910..2676959	-	1050	WP_001265199.1	DUF968 domain-containing protein	-
CPA47_RS13310	2676961..2677239	-	279	WP_012304870.1	hypothetical protein	-
CPA47_RS13315	2677306..2677557	-	252	WP_000980994.1	hypothetical protein	-
CPA47_RS13320	2677774..2677929	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
CPA47_RS13325	2678001..2678288	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
CPA47_RS13330	2678288..2678527	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
CPA47_RS13335	2678552..2678857	+	306	WP_001326990.1	hypothetical protein	-
CPA47_RS13340	2679060..2679392	+	333	WP_001301033.1	protein FlxA	-
CPA47_RS13345	2679829..2681142	-	1314	WP_000589005.1	ISNCY family transposase	-
CPA47_RS13350	2681626..2682654	-	1029	WP_001345551.1	DUF4868 domain-containing protein	-
CPA47_RS13355	2682651..2683265	-	615	WP_001265627.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2645108..2695752	50644
	-	flank	IS/Tn	-	-	2673085..2673543	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T74765 WP_000323025.1 NZ_CP020495:c2678288-2678001 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T74765 NZ_CP020495:c2678288-2678001 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT74765 WP_000534858.1 NZ_CP020495:c2678527-2678288 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT74765 NZ_CP020495:c2678527-2678288 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8