Detailed information of TA system TA074748

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 34763..35871
• Replicon: plasmid unnamed2
• Accession: NZ_CP020490
• Organism: Enterococcus faecium strain CFSAN059071

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: B6S06_RS16290
• Protein ID: WP_000233000.1
• Coordinates: 35002..35871 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: B6S06_RS16285
• Protein ID: WP_000205227.1
• Coordinates: 34763..34987 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6S06_RS16250	30558..31454	+	897	WP_002326827.1	ParA family protein	-
B6S06_RS16255	31552..31761	+	210	WP_000527318.1	peptide-binding protein	-
B6S06_RS16260	31779..32051	+	273	WP_002326825.1	antitoxin	-
B6S06_RS16265	32053..32916	+	864	WP_002332783.1	toxin zeta	-
B6S06_RS16270	33479..33619	+	141	Protein_40	IS6 family transposase	-
B6S06_RS16275	33694..34374	+	681	WP_001067799.1	IS6-like element IS1216 family transposase	-
B6S06_RS16280	34429..34620	+	192	Protein_42	recombinase family protein	-
B6S06_RS16285	34763..34987	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
B6S06_RS16290	35002..35871	+	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
B6S06_RS16295	35852..36586	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
B6S06_RS16300	36619..37527	+	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
B6S06_RS16305	37524..38004	+	481	Protein_47	GNAT family N-acetyltransferase	-
B6S06_RS16310	38093..38773	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
B6S06_RS16315	38982..39194	+	213	WP_002349227.1	hypothetical protein	-
B6S06_RS16320	39356..39874	+	519	WP_000357244.1	YfbU family protein	-
B6S06_RS16325	39881..40393	+	513	WP_000774078.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	VanHAX / cat(pC221) / erm(B) / ant(6)-Ia	-	1..49542	49542
	-	inside	IScluster/Tn	cat(pC221) / erm(B) / ant(6)-Ia	-	22484..47988	25504

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T74748 WP_000233000.1 NZ_CP020490:35002-35871 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T74748 NZ_CP020490:35002-35871 [Enterococcus faecium]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATCGTATTAGGGGGCTC
ACGTGCAAGAGGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAATTGGATGATAAAAATAGAAACAACCTTGTTGTGCCTCCCGGAGCGTGGGGTGATTGG
ATTAATGGCGGCGGATGGTTATTTATAAATGGGTATCATGTTGACTTGATTTTACGTGATATAAAACGGGTAGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCAATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGTAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGCATTAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAATATAAACCTGAAAAATATGCCGAA
AGAGTTAATCATATTTTTGAAGTACTCGGTCTTTCTCTTTTTGAATGCTACGATATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT74748 WP_000205227.1 NZ_CP020490:34763-34987 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT74748 NZ_CP020490:34763-34987 [Enterococcus faecium]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTCAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGAAGAAATAAAAATATCTAACGAATAA

Structures

Toxin

• AlphaFold DB: R3JIN1