Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 31779..32916 Replicon plasmid unnamed2
Accession NZ_CP020490
Organism Enterococcus faecium strain CFSAN059071

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag B6S06_RS16265 Protein ID WP_002332783.1
Coordinates 32053..32916 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag B6S06_RS16260 Protein ID WP_002326825.1
Coordinates 31779..32051 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B6S06_RS16620 27646..27744 + 99 WP_176698583.1 23S rRNA methyltransferase attenuation leader peptide -
B6S06_RS16230 27869..28606 + 738 WP_001038795.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
B6S06_RS16240 28776..29036 + 261 Protein_34 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
B6S06_RS16245 29107..30446 + 1340 WP_086953915.1 IS3-like element ISEnfa3 family transposase -
B6S06_RS16250 30558..31454 + 897 WP_002326827.1 ParA family protein -
B6S06_RS16255 31552..31761 + 210 WP_000527318.1 peptide-binding protein -
B6S06_RS16260 31779..32051 + 273 WP_002326825.1 antitoxin Antitoxin
B6S06_RS16265 32053..32916 + 864 WP_002332783.1 toxin zeta Toxin
B6S06_RS16270 33479..33619 + 141 Protein_40 IS6 family transposase -
B6S06_RS16275 33694..34374 + 681 WP_001067799.1 IS6-like element IS1216 family transposase -
B6S06_RS16280 34429..34620 + 192 Protein_42 recombinase family protein -
B6S06_RS16285 34763..34987 + 225 WP_000205227.1 helix-turn-helix transcriptional regulator -
B6S06_RS16290 35002..35871 + 870 WP_000233000.1 nucleotidyltransferase domain-containing protein -
B6S06_RS16295 35852..36586 + 735 WP_000662263.1 class I SAM-dependent methyltransferase -
B6S06_RS16300 36619..37527 + 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX / cat(pC221) / erm(B) / ant(6)-Ia - 1..49542 49542
- inside IScluster/Tn cat(pC221) / erm(B) / ant(6)-Ia - 22484..47988 25504


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T74747 WP_002332783.1 NZ_CP020490:32053-32916 [Enterococcus faecium]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T74747 NZ_CP020490:32053-32916 [Enterococcus faecium]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGATCAGGTAAAACTAGTTTGCGATCGGCAATTT
CCGAAGAGACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAATACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
AGATAAAGGGTATAATTTAGTGATTGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGATTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGGTATGAA
ACTATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGGATTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT74747 WP_002326825.1 NZ_CP020490:31779-32051 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp

>AT74747 NZ_CP020490:31779-32051 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGACAAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References