Detailed information of TA system TA074740

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 143690..144297
• Replicon: plasmid unnamed1
• Accession: NZ_CP020485
• Organism: Enterococcus faecium strain CFSAN059070

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: B6S05_RS15615
• Protein ID: WP_002321032.1
• Coordinates: 143947..144297 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: B6S05_RS15610
• Protein ID: WP_002287514.1
• Coordinates: 143690..143953 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6S05_RS15560	138726..139625	+	900	WP_002322787.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
B6S05_RS15565	139628..140113	+	486	WP_002296241.1	single-stranded DNA-binding protein	-
B6S05_RS15570	140479..140739	+	261	WP_002296240.1	hypothetical protein	-
B6S05_RS15580	141180..141386	+	207	WP_002296239.1	hypothetical protein	-
B6S05_RS15585	141386..141637	+	252	WP_002296238.1	hypothetical protein	-
B6S05_RS15590	141652..142089	+	438	WP_002296237.1	hypothetical protein	-
B6S05_RS15595	142082..142789	+	708	WP_002353391.1	hypothetical protein	-
B6S05_RS15605	143170..143349	+	180	WP_002305761.1	hypothetical protein	-
B6S05_RS15610	143690..143953	+	264	WP_002287514.1	hypothetical protein	Antitoxin
B6S05_RS15615	143947..144297	+	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B6S05_RS15620	144321..144935	-	615	WP_010729795.1	recombinase family protein	-
B6S05_RS15625	145249..145671	+	423	WP_002288787.1	hypothetical protein	-
B6S05_RS15630	145681..145884	+	204	WP_002299573.1	hypothetical protein	-
B6S05_RS15635	146095..146679	+	585	WP_002299575.1	recombinase family protein	-
B6S05_RS15640	146868..147548	-	681	WP_010861579.1	IS6-like element IS1216 family transposase	-
B6S05_RS15645	147602..148312	-	711	WP_010729797.1	GntR family transcriptional regulator	-
B6S05_RS15650	148296..149030	-	735	WP_010729798.1	GntR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..217014	217014

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T74740 WP_002321032.1 NZ_CP020485:143947-144297 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T74740 NZ_CP020485:143947-144297 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT74740 WP_002287514.1 NZ_CP020485:143690-143953 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT74740 NZ_CP020485:143690-143953 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0