Detailed information of TA system TA074736

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): FicTA/Fic(toxin)
• Location: 5322..6265
• Replicon: plasmid pHELV-2
• Accession: NZ_CP020480
• Organism: Campylobacter helveticus strain ATCC 51209

Toxin (Protein)

• Gene name: Fic
• Uniprot ID: -
• Locus tag: CHELV3228_RS09765
• Protein ID: WP_082200908.1
• Coordinates: 5322..6026 (-)
• Length: 235 a.a.

Antitoxin (Protein)

• Gene name: Fti
• Uniprot ID: -
• Locus tag: CHELV3228_RS09770
• Protein ID: WP_082200884.1
• Coordinates: 6026..6265 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHELV3228_RS09730	559..2223	+	1665	WP_082200877.1	relaxase/mobilization nuclease domain-containing protein	-
CHELV3228_RS09735	2227..2682	+	456	WP_082200878.1	hypothetical protein	-
CHELV3228_RS09745	3044..3871	-	828	WP_082200880.1	hypothetical protein	-
CHELV3228_RS09750	3920..4237	-	318	WP_082200881.1	hypothetical protein	-
CHELV3228_RS09755	4234..4482	-	249	WP_082200882.1	hypothetical protein	-
CHELV3228_RS09760	4534..5193	-	660	WP_082200883.1	ParA family protein	-
CHELV3228_RS09765	5322..6026	-	705	WP_082200908.1	Fic family protein	Toxin
CHELV3228_RS09770	6026..6265	-	240	WP_082200884.1	hypothetical protein	Antitoxin
CHELV3228_RS09775	6305..6727	-	423	WP_082200885.1	vapd	-
CHELV3228_RS09780	6724..7053	-	330	WP_004276316.1	hypothetical protein	-
CHELV3228_RS09785	7319..8164	-	846	WP_082200886.1	hypothetical protein	-
CHELV3228_RS09790	8164..10335	-	2172	WP_082200887.1	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
CHELV3228_RS09795	10433..11053	-	621	WP_082200888.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..34012	34012

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (53-157)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 235 a.a.
• Molecular weight: 27255.21 Da
• Isoelectric Point: 7.4007

>T74736 WP_082200908.1 NZ_CP020480:c6026-5322 [Campylobacter helveticus]
MWDTQDTYTDIDTFLLSTNKVNATSLSDLEHKERILAQLAMQELSKNEIKGNFNYEHLKAIHQFIFKDIYTWAGKDRYEE
KFFKALHKGDSQFCVGVLIPQESKRIFDGLMEKELLKTCKNDEQFIKELANFMADLNAMHPFCEGNGRTQRIFINQLAKN
AGYELDLNLTPKEIMLRASIEAMNHQNSSLEKIIKNNLKKIDMSKQREIEAMQEAVRKASQNLKPKDKSKDLDR

Nucleotide

• Length: 705 bp

>T74736 NZ_CP020480:c6026-5322 [Campylobacter helveticus]
ATGTGGGATACCCAAGATACATATACGGATATTGATACATTTTTACTCTCCACAAATAAGGTTAATGCAACTTCATTAAG
TGATTTAGAACACAAAGAAAGAATACTCGCTCAACTAGCAATGCAAGAACTCAGCAAAAATGAAATAAAAGGTAATTTTA
ACTACGAACACCTAAAAGCAATTCATCAATTTATTTTTAAAGATATTTACACTTGGGCTGGAAAAGACAGATATGAAGAA
AAATTTTTTAAGGCTTTACATAAAGGCGATTCTCAATTTTGTGTTGGCGTTTTAATCCCACAAGAATCAAAAAGAATATT
TGATGGTTTAATGGAAAAAGAATTGCTAAAAACTTGCAAAAATGATGAACAATTTATCAAAGAATTGGCAAATTTTATGG
CTGATTTAAACGCTATGCATCCATTTTGCGAGGGTAATGGACGCACTCAAAGAATTTTTATTAATCAACTTGCAAAAAAT
GCTGGATATGAACTTGATTTGAACTTAACTCCAAAAGAAATTATGCTTCGAGCTTCTATTGAAGCAATGAATCATCAAAA
TAGCTCTTTAGAAAAAATAATCAAAAACAATTTAAAGAAAATTGATATGTCTAAACAGAGGGAAATCGAAGCGATGCAAG
AAGCGGTTAGAAAAGCTTCGCAAAATCTTAAACCTAAAGATAAGAGCAAAGATTTAGATAGATAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9094.37 Da
• Isoelectric Point: 4.6652

>AT74736 WP_082200884.1 NZ_CP020480:c6265-6026 [Campylobacter helveticus]
MLQNCKEVYSLEESLNILNKYANNLTPRQYDNIKSIICSFAIENMHLNEEDILNGIAIQKGDKSVLEITQNYKAQWGCL

Nucleotide

• Length: 240 bp

>AT74736 NZ_CP020480:c6265-6026 [Campylobacter helveticus]
ATGTTGCAAAATTGTAAAGAAGTTTATAGCTTAGAAGAAAGCCTAAATATTTTAAATAAATATGCAAATAATCTCACACC
AAGACAATACGATAATATTAAGTCTATTATATGTAGCTTTGCCATAGAAAATATGCATCTCAATGAAGAAGATATTTTAA
ACGGCATAGCCATTCAAAAAGGCGATAAGAGTGTTTTAGAAATTACTCAAAATTATAAAGCTCAGTGGGGTTGCCTTTAA