Detailed information of TA system TA074707

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3402110..3402702
• Replicon: chromosome
• Accession: NZ_CP020466
• Organism: Edwardsiella ictaluri strain RUSVM-1

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: D0ZEC2
• Locus tag: B6E78_RS15850
• Protein ID: WP_012847872.1
• Coordinates: 3402110..3402313 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: B6E78_RS15855
• Protein ID: WP_081168012.1
• Coordinates: 3402334..3402702 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6E78_RS15810	3397330..3397791	+	462	WP_015870480.1	Lrp/AsnC family transcriptional regulator	-
B6E78_RS17310	3397820..3398035	-	216	WP_128574010.1	hypothetical protein	-
B6E78_RS15815	3398090..3398428	+	339	WP_015870482.1	P-II family nitrogen regulator	-
B6E78_RS15820	3398450..3399730	+	1281	WP_049640187.1	ammonium transporter AmtB	-
B6E78_RS15825	3399764..3400630	-	867	WP_015870484.1	acyl-CoA thioesterase II	-
B6E78_RS15830	3400676..3401005	-	330	WP_015870485.1	MGMT family protein	-
B6E78_RS17315	3401983..3402090	+	108	Protein_3033	IS5/IS1182 family transposase	-
B6E78_RS15850	3402110..3402313	-	204	WP_012847872.1	hemolysin expression modulator Hha	Toxin
B6E78_RS15855	3402334..3402702	-	369	WP_081168012.1	Hha toxicity modulator TomB	Antitoxin
B6E78_RS15860	3403062..3406214	-	3153	WP_015870488.1	multidrug efflux RND transporter permease subunit	-
B6E78_RS15865	3406258..3407442	-	1185	WP_081168015.1	efflux RND transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-115)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8103.37 Da
• Isoelectric Point: 5.0914

>T74707 WP_012847872.1 NZ_CP020466:c3402313-3402110 [Edwardsiella ictaluri]
MTKIDYLMKLRKCTTLDTLERVIEKNKYELSDDELEIFYSAADHRLAELTMNKLYDKIPAEVWQYVR

Nucleotide

• Length: 204 bp

>T74707 NZ_CP020466:c3402313-3402110 [Edwardsiella ictaluri]
ATGACAAAAATCGATTATCTGATGAAGTTGCGTAAATGCACGACGCTGGATACTCTGGAACGCGTGATCGAAAAGAACAA
ATACGAACTTTCAGATGATGAGCTGGAAATTTTTTATTCTGCCGCAGATCATCGACTGGCTGAATTAACCATGAATAAAC
TATACGACAAAATTCCGGCAGAAGTTTGGCAATACGTCCGCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14259.02 Da
• Isoelectric Point: 5.4786

>AT74707 WP_081168012.1 NZ_CP020466:c3402702-3402334 [Edwardsiella ictaluri]
MDENTSYQHDISELKYLCDYLYHQGIDVLGESNHGWVSDPTAEVNLQLNELIEHIASIAQSFKIKYPRHSDLAEMLDYYL
DETYALFGTYSISETALKQWLRTKRRMAYCLAHEKRNAALHV

Nucleotide

• Length: 369 bp

>AT74707 NZ_CP020466:c3402702-3402334 [Edwardsiella ictaluri]
ATGGATGAGAACACTTCTTATCAGCATGACATCTCAGAGTTAAAATACCTGTGTGATTACCTGTATCATCAGGGAATAGA
TGTCTTGGGAGAAAGCAATCACGGCTGGGTCAGCGATCCGACGGCAGAAGTAAATCTACAGCTTAACGAGCTGATTGAGC
ATATTGCCAGCATCGCGCAATCATTTAAGATCAAATATCCGCGTCATTCGGATTTAGCCGAAATGCTGGATTATTATCTG
GATGAAACCTATGCCCTGTTTGGCACCTATAGCATAAGTGAAACGGCGCTCAAGCAATGGTTGCGTACCAAACGTCGGAT
GGCATACTGTCTGGCGCATGAAAAGCGCAACGCTGCCTTACATGTTTAG

Structures

Toxin

• AlphaFold DB: A0A034SMG4