Detailed information of TA system TA074706

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 1795378..1796041
• Replicon: chromosome
• Accession: NZ_CP020466
• Organism: Edwardsiella ictaluri strain RUSVM-1

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: C5BAS3
• Locus tag: B6E78_RS08410
• Protein ID: WP_015872556.1
• Coordinates: 1795378..1795794 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: C5BAS4
• Locus tag: B6E78_RS08415
• Protein ID: WP_015872557.1
• Coordinates: 1795775..1796041 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6E78_RS08390	1791301..1793034	-	1734	WP_081166575.1	single-stranded-DNA-specific exonuclease RecJ	-
B6E78_RS08395	1793039..1793755	-	717	WP_081166578.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
B6E78_RS08400	1793783..1794682	-	900	WP_081166581.1	site-specific tyrosine recombinase XerD	-
B6E78_RS08405	1794841..1795353	+	513	WP_015872555.1	flavodoxin FldB	-
B6E78_RS08410	1795378..1795794	-	417	WP_015872556.1	protein YgfX	Toxin
B6E78_RS08415	1795775..1796041	-	267	WP_015872557.1	FAD assembly factor SdhE	Antitoxin
B6E78_RS08420	1796395..1797390	+	996	WP_015872558.1	tRNA-modifying protein YgfZ	-
B6E78_RS08425	1797431..1798255	-	825	WP_081166585.1	shikimate 5-dehydrogenase	-
B6E78_RS08430	1798417..1799289	-	873	WP_015872560.1	nucleoside-specific channel-forming protein Tsx	-
B6E78_RS08435	1799894..1800544	+	651	WP_035609190.1	hemolysin III family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 16145.89 Da
• Isoelectric Point: 11.3233

>T74706 WP_015872556.1 NZ_CP020466:c1795794-1795378 [Edwardsiella ictaluri]
VALWRCNVHASWRTQVMFLLGHGALVLAILLAPWPENYSVFWLILLVAVIFECIRSQRLIARNSGELQRLAPQVWHWQLR
EWRLARRPWVSDLGALLILQSTHGTPCRRRLWLAADSMSREEWGQLRRALLDTGDDRV

Nucleotide

• Length: 417 bp

>T74706 NZ_CP020466:c1795794-1795378 [Edwardsiella ictaluri]
GTGGCCCTTTGGCGCTGTAATGTTCACGCCTCCTGGCGTACCCAGGTGATGTTTCTGCTGGGGCATGGCGCGCTGGTATT
GGCGATCCTGTTGGCGCCCTGGCCGGAAAACTACAGCGTATTCTGGCTGATTCTGCTGGTTGCGGTGATCTTTGAGTGTA
TCCGCAGCCAGCGTCTGATTGCGCGTAACAGCGGTGAGCTACAGCGGCTGGCCCCACAGGTATGGCACTGGCAGCTGCGC
GAGTGGCGCCTGGCCCGGCGCCCCTGGGTTAGCGATCTGGGAGCGTTGCTGATCCTGCAGAGTACCCATGGCACGCCGTG
CCGCCGTCGTCTGTGGCTGGCGGCCGACAGCATGAGCCGCGAAGAGTGGGGGCAGCTGCGCCGCGCGCTGCTCGATACGG
GCGACGATCGCGTATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10364.92 Da
• Isoelectric Point: 5.6951

>AT74706 WP_015872557.1 NZ_CP020466:c1796041-1795775 [Edwardsiella ictaluri]
MDINNKARVHWACRRGMRELDIAIMPFFEHEYDALNDADKQTFVRLLSCDDPELFKWLMNQGKPDDTDLARMITLIQTRN
KERGPLAL

Nucleotide

• Length: 267 bp

>AT74706 NZ_CP020466:c1796041-1795775 [Edwardsiella ictaluri]
ATGGATATCAATAATAAAGCGCGGGTTCACTGGGCGTGCCGCCGCGGAATGCGTGAGCTGGATATCGCCATTATGCCTTT
CTTTGAACATGAGTATGATGCGCTTAACGATGCCGATAAACAGACCTTTGTCCGACTGCTGTCCTGTGACGATCCTGAAC
TATTTAAATGGCTGATGAACCAGGGTAAGCCGGACGATACCGATCTGGCCCGTATGATTACGCTGATTCAGACGCGAAAT
AAAGAACGTGGCCCTTTGGCGCTGTAA

Structures

Toxin

• AlphaFold DB: C5BAS3

Antitoxin

• AlphaFold DB: C5BAS4