Detailed information of TA system TA074701

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 547850..548501
• Replicon: chromosome
• Accession: NZ_CP020464
• Organism: Lacticaseibacillus rhamnosus strain Pen

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: K8Q4Y0
• Locus tag: BVH57_RS02750
• Protein ID: WP_005686631.1
• Coordinates: 548118..548501 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: BVH57_RS02745
• Protein ID: WP_081155001.1
• Coordinates: 547850..548098 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVH57_RS02725	542899..544287	+	1389	WP_005714110.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
BVH57_RS02730	544561..546069	+	1509	WP_005686635.1	DEAD/DEAH box helicase	-
BVH57_RS02735	546238..546612	+	375	WP_005686634.1	holo-ACP synthase	-
BVH57_RS02740	546599..547738	+	1140	WP_005710798.1	alanine racemase	-
BVH57_RS02745	547850..548098	+	249	WP_081155001.1	antitoxin	Antitoxin
BVH57_RS02750	548118..548501	+	384	WP_005686631.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BVH57_RS02755	548498..548711	-	214	Protein_507	Malolactic regulator	-
BVH57_RS02760	548678..548875	+	198	WP_080565105.1	hypothetical protein	-
BVH57_RS02765	548913..549440	+	528	WP_005711058.1	QueT transporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13757.03 Da
• Isoelectric Point: 10.9764

>T74701 WP_005686631.1 NZ_CP020464:548118-548501 [Lacticaseibacillus rhamnosus]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Nucleotide

• Length: 384 bp

>T74701 NZ_CP020464:548118-548501 [Lacticaseibacillus rhamnosus]
ATGGATGTAACAGTCAAACGCGGGGATGTATTTTTCGCCGATTTATCGCCGGTTGTCGGGTCGGAACAAGGCGGTAACCG
GCCCGTACTTATTATTCAAAACAATGTTGGTAACCATTATAGCCCCACTGTTATCGTGGCTGCCATTACCTCCAAAATCC
AAAAACCCAAAATGCCCACCCATGTCGGTTTGCGCGCCAAGCAAGACGGGGTTGAGCGTAATTCAGTCATTTTGCTTGAA
CAGATTCGTACAATTGATAAGCAACGGCTACAGGCACGCGTTACCGCTCTCTCAAGTGCCAAAATGGCAGCGGTGGATCG
GGCACTGGCGATATCGGTCGGACTCGTCAGCTTGCCTAAGCCGAAAACATATAATAAAAACTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8980.17 Da
• Isoelectric Point: 4.4778

>AT74701 WP_081155001.1 NZ_CP020464:547850-548098 [Lacticaseibacillus rhamnosus]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSAFESEAYAH
IR

Nucleotide

• Length: 249 bp

>AT74701 NZ_CP020464:547850-548098 [Lacticaseibacillus rhamnosus]
ATGAAGAAGGAAACGTCAGTAACGGTCGCGCTTGATAGTGCTATCAAAGCGCGTTTGGATGCCTATTGCGAGTTGAATGA
TATGGGTGTTCAGGATGTCATTACTGAAGCAGTGGCGAAGTTTTTGCAGTCACAAGCACCTGAGATCAATCAATTGATTT
CTGGCTATGTGGCAATGGGACAAATCAATACGGAAATCAGCCATGCTTTCTCCGCCTTCGAAAGTGAGGCATACGCCCAT
ATTCGATAA

Structures

Toxin

• AlphaFold DB: K8Q4Y0