Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2688455..2689097 Replicon chromosome
Accession NZ_CP020437
Organism Bacillus sp. FDAARGOS_235

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag A6J74_RS15615 Protein ID WP_000635963.1
Coordinates 2688455..2688805 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag A6J74_RS15620 Protein ID WP_000004570.1
Coordinates 2688810..2689097 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A6J74_RS15600 2685368..2685835 - 468 WP_000544525.1 SprT family protein -
A6J74_RS15605 2686043..2686159 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
A6J74_RS15610 2686218..2688386 - 2169 WP_000426215.1 RNA-binding transcriptional accessory protein -
A6J74_RS15615 2688455..2688805 - 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
A6J74_RS15620 2688810..2689097 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
A6J74_RS15625 2689397..2690566 - 1170 WP_000390621.1 alanine racemase -
A6J74_RS15630 2690684..2691634 - 951 WP_002037834.1 outer membrane lipoprotein carrier protein LolA -
A6J74_RS15635 2691791..2692150 - 360 WP_000635048.1 holo-ACP synthase -
A6J74_RS15640 2692243..2692815 + 573 WP_000908503.1 rhomboid family intramembrane serine protease -
A6J74_RS15645 2692808..2693770 - 963 WP_000961174.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T74683 WP_000635963.1 NZ_CP020437:c2688805-2688455 [Bacillus sp. FDAARGOS_235]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T74683 NZ_CP020437:c2688805-2688455 [Bacillus sp. FDAARGOS_235]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGATTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGATTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT74683 WP_000004570.1 NZ_CP020437:c2689097-2688810 [Bacillus sp. FDAARGOS_235]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT74683 NZ_CP020437:c2689097-2688810 [Bacillus sp. FDAARGOS_235]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACGGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References