Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 3758326..3758881 Replicon chromosome
Accession NZ_CP020414
Organism Leptospira interrogans serovar Copenhageni strain FDAARGOS_203

Toxin (Protein)


Gene name chpK Uniprot ID Q72T28
Locus tag A6J42_RS18005 Protein ID WP_000617907.1
Coordinates 3758561..3758881 (+) Length 107 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID Q93MT9
Locus tag A6J42_RS18000 Protein ID WP_000844758.1
Coordinates 3758326..3758574 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A6J42_RS17985 3753978..3755960 + 1983 WP_000802798.1 GAF domain-containing protein -
A6J42_RS20715 3756602..3757060 - 459 WP_000453263.1 hypothetical protein -
A6J42_RS17995 3757214..3757510 + 297 WP_000477061.1 helix-turn-helix domain-containing protein -
A6J42_RS18000 3758326..3758574 + 249 WP_000844758.1 ribbon-helix-helix domain-containing protein Antitoxin
A6J42_RS18005 3758561..3758881 + 321 WP_000617907.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
A6J42_RS18010 3759881..3760873 - 993 WP_000041092.1 aldolase -
A6J42_RS18015 3761013..3761948 - 936 WP_000004530.1 ornithine carbamoyltransferase -
A6J42_RS18025 3762752..3763219 + 468 WP_000060053.1 hypothetical protein -
A6J42_RS18030 3763216..3763620 + 405 WP_001974506.1 pyridoxamine 5'-phosphate oxidase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-103)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 107 a.a.        Molecular weight: 11644.43 Da        Isoelectric Point: 9.8447

>T74660 WP_000617907.1 NZ_CP020414:3758561-3758881 [Leptospira interrogans serovar Copenhageni]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGKVEIGL

Download         Length: 321 bp

>T74660 NZ_CP020414:3758561-3758881 [Leptospira interrogans serovar Copenhageni]
ATGATTCGTGGTGAAATTTGGTGGGTAGACTTGGGAATTCCATTTGGAAGTGAGCCTGGATTTAAACGTCCAGTTCTTAT
AATTCAAGATGACTCTTTTAATCAAAGTAATATTAATACAATAGTTTCGATTGCAATTACATCGAATTTAAATCTATCTG
AAGCACCAGGTAATGTTTTCATTAGTAAAAAAGATTCGAGTTTATCAAAGGACTCGGTCATAAATGTCTCTCAAATTGTG
ACTTTAGATAAAGAAAGATTTCTTAACAAAGCGGGTAAACTTAAATCAAATAAATTAGGTAAAGTTGAAATTGGGCTTTA
G

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9380.77 Da        Isoelectric Point: 10.3815

>AT74660 WP_000844758.1 NZ_CP020414:3758326-3758574 [Leptospira interrogans serovar Copenhageni]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT74660 NZ_CP020414:3758326-3758574 [Leptospira interrogans serovar Copenhageni]
ATGAAGACGGCGATTTCAATTCCTGATGAGTTATTTAGGGCTGCCGAGAAAATAGCTAAAAAACTTGGGATTCCTCGAAG
CCAGTTATTTGCAAAAGCATTAGAAGAATTTATCCAATCTCATAGTAAAGAATCTGTAACTGAGAAATTAAATAAAATCT
ACAGTAATAAATCCAAAGAAACTAGAAATAATATTACTGACTTATCCGTAGAATCGCTTCGTAAGAGTTTAAAAAATGAT
TCGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q72T28

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HD92

References