Detailed information of TA system TA074658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1737348..1737985
• Replicon: chromosome
• Accession: NZ_CP020411
• Organism: Haemophilus influenzae strain FDAARGOS_199

Toxin (Protein)

• Gene name: VapC1
• Uniprot ID: E4QWH2
• Locus tag: A6J38_RS08625
• Protein ID: WP_005649049.1
• Coordinates: 1737348..1737752 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: E4QWH3
• Locus tag: A6J38_RS08630
• Protein ID: WP_005649046.1
• Coordinates: 1737749..1737985 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6J38_RS08595	1732908..1733474	+	567	WP_005544066.1	elongation factor P	-
A6J38_RS08605	1733825..1734412	-	588	WP_005654063.1	primosomal replication protein	-
A6J38_RS08610	1734445..1735797	-	1353	WP_005694344.1	Na+/H+ antiporter family protein	-
A6J38_RS08615	1736111..1736800	-	690	WP_005694345.1	ribonuclease T	-
A6J38_RS08620	1736874..1737281	-	408	WP_005688980.1	lactoylglutathione lyase	-
A6J38_RS08625	1737348..1737752	-	405	WP_005649049.1	type II toxin-antitoxin system VapC family toxin	Toxin
A6J38_RS08630	1737749..1737985	-	237	WP_005649046.1	antitoxin	Antitoxin
A6J38_RS08635	1738079..1738804	-	726	WP_005694346.1	carboxy-S-adenosyl-L-methionine synthase CmoA	-
A6J38_RS08640	1738857..1739375	-	519	WP_005649040.1	isoprenylcysteine carboxyl methyltransferase family protein	-
A6J38_RS08645	1739594..1741360	+	1767	WP_005694347.1	aspartate--tRNA ligase	-
A6J38_RS08650	1741382..1741858	+	477	WP_005694349.1	dihydroneopterin triphosphate diphosphatase	-
A6J38_RS08655	1742018..1742758	+	741	WP_005649034.1	YebC/PmpR family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• SpoVT-AbrB domain (6-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15692.29 Da
• Isoelectric Point: 8.9777

>T74658 WP_005649049.1 NZ_CP020411:c1737752-1737348 [Haemophilus influenzae]
MIYMLDTNIIIYLMKNRPKIIAERVSQLLPNDRLVMSFITYAELIKGAFGSQNYEQSIRAIELLTERVNVLYPNEQICLH
YGKWANTLKKQGRPIGNNDLWIACHALSLNAVLITHNVKEFQRITDLQWQDWTK

Nucleotide

• Length: 405 bp

>T74658 NZ_CP020411:c1737752-1737348 [Haemophilus influenzae]
ATGATTTATATGTTAGACACCAATATCATTATTTATTTAATGAAAAATCGCCCCAAAATTATTGCCGAACGAGTATCACA
ATTATTGCCTAATGATCGCTTAGTTATGAGCTTTATTACTTATGCTGAACTTATTAAAGGCGCCTTTGGTAGTCAAAATT
ATGAGCAATCAATACGAGCAATAGAATTACTTACTGAACGAGTGAATGTACTATATCCCAATGAACAAATCTGTTTACAT
TATGGCAAATGGGCAAATACACTCAAAAAACAAGGGCGACCTATCGGAAATAATGATCTATGGATCGCTTGTCACGCATT
GAGTTTAAATGCCGTTCTTATTACACATAATGTAAAAGAATTTCAGCGAATTACAGATCTTCAGTGGCAAGATTGGACAA
AATAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9037.19 Da
• Isoelectric Point: 4.2342

>AT74658 WP_005649046.1 NZ_CP020411:c1737985-1737749 [Haemophilus influenzae]
MLTKVFQSGNSQAVRIPMDFRFDVDTVEIFRKENGDVVLRPVSKKTDDFLALFEGFDETFIQALEARDDLPPQERENL

Nucleotide

• Length: 237 bp

>AT74658 NZ_CP020411:c1737985-1737749 [Haemophilus influenzae]
ATGCTTACTAAAGTGTTTCAAAGTGGTAACAGCCAAGCTGTTCGGATCCCGATGGACTTTCGTTTTGACGTCGATACCGT
AGAAATTTTCCGAAAGGAAAATGGGGATGTGGTATTACGCCCAGTTTCTAAAAAAACAGATGATTTTCTTGCGTTATTTG
AAGGATTTGATGAGACCTTTATTCAAGCACTTGAAGCGCGTGATGATTTACCGCCTCAGGAGCGAGAAAATTTATGA

Structures

Toxin

• AlphaFold DB: E4QWH2

Antitoxin

• AlphaFold DB: E4QWH3