Detailed information of TA system TA074611

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 655070..655588
• Replicon: chromosome
• Accession: NZ_CP020406
• Organism: Staphylococcus lugdunensis strain FDAARGOS_222

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: -
• Locus tag: A6J61_RS03055
• Protein ID: WP_002478973.1
• Coordinates: 655376..655588 (+)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: A0A853V130
• Locus tag: A6J61_RS03050
• Protein ID: WP_002492098.1
• Coordinates: 655070..655363 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6J61_RS03030	650676..651314	+	639	WP_002461213.1	thiamine phosphate synthase	-
A6J61_RS03035	651404..652279	+	876	WP_002461212.1	membrane protein insertase YidC	-
A6J61_RS03040	652657..653298	-	642	WP_070647978.1	HD domain-containing protein	-
A6J61_RS03045	653420..654904	-	1485	WP_070647977.1	cardiolipin synthase	-
A6J61_RS03050	655070..655363	+	294	WP_002492098.1	copper-sensing transcriptional repressor CsoR	Antitoxin
A6J61_RS03055	655376..655588	+	213	WP_002478973.1	heavy-metal-associated domain-containing protein	Toxin
A6J61_RS03060	655679..655816	+	138	WP_002461206.1	hypothetical protein	-
A6J61_RS03065	656230..657420	-	1191	WP_002461205.1	rod shape-determining protein RodA	-
A6J61_RS03070	657776..658846	+	1071	WP_002461204.1	D-alanine--D-alanine ligase	-
A6J61_RS03075	658864..660222	+	1359	WP_062077884.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (19-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-90)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 8070.03 Da
• Isoelectric Point: 3.9947

>T74611 WP_002478973.1 NZ_CP020406:655376-655588 [Staphylococcus lugdunensis]
VEKRLIYITNVTSEDDLENLRTRLLEMIGVNEVVINFDERAVNVTFETPTNLNALEKEIYDAGYHAIDDL

Nucleotide

• Length: 213 bp

>T74611 NZ_CP020406:655376-655588 [Staphylococcus lugdunensis]
GTGGAAAAGCGATTAATTTATATTACAAATGTGACTAGTGAAGACGACTTGGAAAACTTACGAACAAGGTTATTAGAGAT
GATTGGTGTTAATGAAGTAGTGATCAATTTTGATGAACGTGCAGTTAATGTTACTTTTGAAACACCAACTAATTTAAATG
CACTTGAAAAAGAAATATATGATGCTGGCTATCATGCTATAGATGATTTATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11177.85 Da
• Isoelectric Point: 6.6367

>AT74611 WP_002492098.1 NZ_CP020406:655070-655363 [Staphylococcus lugdunensis]
MNEQDHAHHSKIVKANLKSRLNRIEGQVRAINRMVEDDVYCDDVLTQIRATRSALTSVATKLLEHHMKSCIMDKINDGQE
VEAMEELLVTFQKMLKD

Nucleotide

• Length: 294 bp

>AT74611 NZ_CP020406:655070-655363 [Staphylococcus lugdunensis]
TTGAACGAACAAGATCATGCACATCATTCTAAAATTGTAAAAGCAAATTTAAAATCTAGATTAAATCGTATAGAAGGACA
AGTGCGGGCAATTAATCGCATGGTTGAAGATGATGTTTATTGTGATGATGTGTTAACACAAATAAGAGCAACACGCTCAG
CATTAACGAGTGTAGCAACAAAACTATTAGAGCATCATATGAAAAGTTGTATCATGGATAAAATAAACGATGGGCAGGAA
GTTGAAGCGATGGAGGAATTGCTAGTTACATTTCAAAAAATGCTAAAAGATTAG

Structures

Antitoxin

• AlphaFold DB: A0A853V130