Detailed information of TA system TA074609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 2061709..2062310
• Replicon: chromosome
• Accession: NZ_CP020405
• Organism: Pasteurella multocida strain FDAARGOS_218

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A8E2DA97
• Locus tag: A6J57_RS09650
• Protein ID: WP_005756650.1
• Coordinates: 2061709..2062023 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: A6J57_RS09655
• Protein ID: WP_005756647.1
• Coordinates: 2062020..2062310 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6J57_RS09620	2057368..2058624	-	1257	WP_032854019.1	hypothetical protein	-
A6J57_RS09625	2058707..2059504	-	798	WP_032854018.1	hypothetical protein	-
A6J57_RS09630	2059622..2060086	-	465	WP_050547907.1	DUF4760 domain-containing protein	-
A6J57_RS09635	2060539..2060766	-	228	WP_032854017.1	hypothetical protein	-
A6J57_RS09640	2061009..2061218	-	210	WP_005756656.1	hypothetical protein	-
A6J57_RS09645	2061231..2061398	+	168	WP_005756653.1	DUF1508 domain-containing protein	-
A6J57_RS09650	2061709..2062023	+	315	WP_005756650.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A6J57_RS09655	2062020..2062310	+	291	WP_005756647.1	putative addiction module antidote protein	Antitoxin
A6J57_RS09660	2062334..2063041	-	708	WP_005756645.1	hypothetical protein	-
A6J57_RS09665	2063038..2064105	-	1068	WP_005756643.1	DUF262 domain-containing protein	-
A6J57_RS09670	2064149..2064838	-	690	WP_005756642.1	helix-turn-helix transcriptional regulator	-
A6J57_RS09675	2064966..2065175	+	210	WP_005720263.1	helix-turn-helix transcriptional regulator	-
A6J57_RS09680	2065244..2065471	+	228	WP_014391093.1	hypothetical protein	-
A6J57_RS09685	2065474..2065881	-	408	WP_032854015.1	hypothetical protein	-
A6J57_RS09690	2065988..2066233	+	246	WP_005725517.1	helix-turn-helix domain-containing protein	-
A6J57_RS09695	2066311..2067129	+	819	WP_005756636.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2046416..2107384	60968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-91)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11749.63 Da
• Isoelectric Point: 9.5553

>T74609 WP_005756650.1 NZ_CP020405:2061709-2062023 [Pasteurella multocida]
MLDVIETTVFNKWLKELKDLSAKAAILARISRAKSGNFGDHKSVGDGLYEMRIMKGAGYRVYYAQYKDVTYLLICGGDKS
TQKADIAKAKALWEEIKQKEELNV

Nucleotide

• Length: 315 bp

>T74609 NZ_CP020405:2061709-2062023 [Pasteurella multocida]
ATGCTCGATGTAATTGAAACAACAGTATTTAATAAATGGCTTAAAGAGTTAAAGGATTTGTCGGCTAAGGCGGCTATTCT
TGCTCGTATTAGTCGTGCTAAAAGTGGTAATTTTGGCGATCATAAATCAGTTGGTGATGGTTTGTATGAAATGCGAATAA
TGAAAGGAGCTGGTTATAGGGTTTATTATGCACAATACAAAGATGTAACCTATTTACTAATTTGTGGCGGTGATAAATCT
ACTCAGAAAGCAGATATCGCTAAAGCAAAAGCATTATGGGAAGAGATTAAGCAGAAAGAGGAGCTAAATGTATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10564.25 Da
• Isoelectric Point: 6.2258

>AT74609 WP_005756647.1 NZ_CP020405:2062020-2062310 [Pasteurella multocida]
MSKKVELKPFDIAEHLDSEEMIAAYLSEVLETGDTNEFISALGDVARARGMTELAAKTGLGRESLYKTLSHGSKPRFDTI
MKITQALGIKLVPMHI

Nucleotide

• Length: 291 bp

>AT74609 NZ_CP020405:2062020-2062310 [Pasteurella multocida]
ATGAGTAAAAAAGTAGAATTAAAACCATTTGATATTGCTGAACATCTAGATAGTGAAGAAATGATTGCGGCATATTTAAG
TGAAGTTTTAGAAACAGGTGATACTAATGAATTTATTTCTGCGCTAGGTGATGTCGCAAGAGCAAGAGGAATGACGGAGT
TAGCAGCAAAAACAGGGCTGGGAAGAGAAAGCCTCTATAAAACATTATCACACGGAAGTAAGCCACGTTTTGATACTATA
ATGAAAATTACGCAAGCACTAGGTATTAAACTTGTCCCCATGCACATCTAA