Detailed information of TA system TA074597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1625374..1625955
• Replicon: chromosome
• Accession: NZ_CP020397
• Organism: Burkholderia multivorans strain FDAARGOS_246

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: A8H40_RS08060
• Protein ID: WP_035946056.1
• Coordinates: 1625662..1625955 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: J4SGI3
• Locus tag: A8H40_RS08055
• Protein ID: WP_006409266.1
• Coordinates: 1625374..1625658 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8H40_RS08030	1621022..1622443	-	1422	WP_043279886.1	FAD-binding oxidoreductase	-
A8H40_RS08035	1622462..1622857	-	396	WP_006404970.1	DUF2069 domain-containing protein	-
A8H40_RS08040	1622854..1623465	-	612	WP_006402099.1	NAD(P)H:quinone oxidoreductase	-
A8H40_RS08045	1623610..1624938	+	1329	WP_045594112.1	YihY family inner membrane protein	-
A8H40_RS08050	1625102..1625290	-	189	WP_006409259.1	hypothetical protein	-
A8H40_RS08055	1625374..1625658	-	285	WP_006409266.1	putative addiction module antidote protein	Antitoxin
A8H40_RS08060	1625662..1625955	-	294	WP_035946056.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A8H40_RS08065	1626181..1626498	-	318	WP_006402097.1	DUF962 domain-containing protein	-
A8H40_RS08070	1626659..1627114	+	456	WP_006402096.1	CBS domain-containing protein	-
A8H40_RS08075	1627263..1629197	+	1935	WP_006404968.1	MFS transporter	-
A8H40_RS08080	1629366..1630466	+	1101	WP_006404967.1	chorismate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-88)

Antitoxin

• Uncharacterized protein HI1420 (5-90)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11168.92 Da
• Isoelectric Point: 9.4615

>T74597 WP_035946056.1 NZ_CP020397:c1625955-1625662 [Burkholderia multivorans]
MVTLLRSTEFNDWLANLRDQRGKARIAARLISAQLGNFGEYRVLDEGVCELKIDFGPGYRVYYVRRGEVVYLLLCGGDKS
TQRKDIKPVQMARELKE

Nucleotide

• Length: 294 bp

>T74597 NZ_CP020397:c1625955-1625662 [Burkholderia multivorans]
ATGGTTACTTTGCTGCGATCCACGGAGTTCAACGATTGGCTTGCCAACCTGCGCGATCAGCGCGGCAAGGCCCGAATCGC
AGCCCGCCTGATCAGCGCGCAACTCGGCAACTTCGGCGAGTACCGTGTGCTCGACGAAGGGGTCTGCGAACTGAAGATCG
ATTTCGGTCCGGGCTACCGCGTGTATTACGTTCGACGCGGCGAAGTGGTCTACCTCCTTCTGTGCGGCGGCGACAAGTCA
ACGCAACGGAAGGACATCAAGCCCGTGCAGATGGCGCGCGAACTGAAGGAGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 9986.38 Da
• Isoelectric Point: 6.2121

>AT74597 WP_006409266.1 NZ_CP020397:c1625658-1625374 [Burkholderia multivorans]
MTNYTEFDAADYLDNDEAVAEFLAVAAEDPNPDVFLAAVAAVARARGMTKVAAAAGVGRVALHKALTPGAHPRYETVQKI
IRSLGLRMSFTVAH

Nucleotide

• Length: 285 bp

>AT74597 NZ_CP020397:c1625658-1625374 [Burkholderia multivorans]
ATGACCAACTACACCGAGTTCGATGCGGCCGATTACCTCGACAACGATGAGGCGGTCGCGGAATTCCTCGCCGTCGCGGC
AGAGGACCCGAATCCTGACGTGTTCCTCGCGGCCGTCGCTGCGGTCGCGCGGGCGCGCGGTATGACGAAAGTTGCGGCAG
CCGCCGGCGTCGGACGCGTCGCGCTTCACAAGGCACTGACGCCCGGCGCGCACCCGCGCTACGAAACCGTGCAGAAAATC
ATCCGGTCGCTCGGCCTGCGCATGAGCTTCACGGTCGCGCACTAA

Structures

Antitoxin

• AlphaFold DB: J4SGI3